Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # VIK-10

Format

BSA Free

Concentration

1.0 mg/ml

View all Cytokeratin 10 Antibodies »

Product Specifications

Immunogen

Cytoskeleton preparation extracted from human epidermis by detergent/high salt extraction.

Specificity

The antibody VIK-10 reacts with Cytokeratin 10 (56.5 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cytokeratin 10 Antibody (VIK-10) - BSA Free

Immunohistochemistry: Cytokeratin 10 Antibody (VIK-10) - BSA Free [NB500-354]

Immunohistochemistry: Cytokeratin 10 Antibody (VIK-10) - BSA Free [NB500-354]

Immunohistochemistry: Cytokeratin 10 Antibody (VIK-10) [NB500-354] - Fig. 1. Immunohistochemistry staining of skin basaliom (paraffin-embedded sections) with anti-human Cytokeratin 10 (VIK-10).

Applications for Cytokeratin 10 Antibody (VIK-10) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry-Paraffin

10 ug/ml

Immunoprecipitation

1:50
Application Notes
Immunohistochemistry-Paraffin sections - Pretreatment: trypsin/ temperature 37C

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Cytokeratin 10

Cytokeratin 10 is a heterotetramer of two type I and two type II keratins. Cytokeratin 10 is generally associated with keratin 1. It is seen in all suprabasal cell layers including stratum corneum. A number of alleles are known that mainly differ in the Gly-rich region (positions 490-560). Defects in cytokeratin 10 are a cause of epidermolytic hyperkeratosis (EHK), also known as bullous congenital ichthyosiform erythroderma (BCIE) or bullous erythroderma ichthyosiformis congenita of Brocq. EHK is an hereditary skin disorder characterized by blistering and a marked thickening of the stratum corneum. At birth, affected individuals usually present with redness, blisters and superficial erosions due to cytolysis. Within a few weeks, the erythroderma and blister formation diminish and hyperkeratoses develop. Transmission is autosomal dominant, but most cases are sporadic. Defects in cytokeratin 10 are also a cause of annular epidermolytic ichthyosis (AEI), also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI resembles clinical and histologic features of both epidermolytic hyperkeratosis and ichthyosis bullosa of Siemens.

Alternate Names

BCIE, BIE, CK10, CK-10, cytokeratin 10, Cytokeratin-10, EHK, K10keratosis palmaris et plantaris, keratin 10, Keratin 10 (epidermolytic hyperkeratosis; keratosis palmaris et plantaris), keratin, type I cytoskeletal 10, keratin-10, KPP

Entrez Gene IDs

3858 (Human)

Gene Symbol

KRT10

Additional Cytokeratin 10 Products

Product Documents for Cytokeratin 10 Antibody (VIK-10) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Cytokeratin 10 Antibody (VIK-10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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