Cytokeratin 13 Antibody (4F5)

Novus Biologicals, part of Bio-Techne | Catalog # H00003860-M05

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4F5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Cytokeratin 13 Antibodies »

Product Specifications

Immunogen

KRT13 (NP_705694.1, 258 a.a. ~ 357 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VNVEMDATPGIDLTRVLAEMREQYEAMAERNRRDAEEWFHAKSAELNKEVSTNTAMIQTSKTEITELRRTLQGLEIELQSQLSMKAGLENTVAETECRYA

Specificity

KRT13 - keratin 13 (4F5)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Cytokeratin 13 Antibody (4F5)

Western Blot: Cytokeratin 13 Antibody (4F5) [H00003860-M05]

Western Blot: Cytokeratin 13 Antibody (4F5) [H00003860-M05]

Western Blot: Cytokeratin 13 Antibody (4F5) [H00003860-M05] - Analysis of KRT13 expression in transfected 293T cell line by KRT13 monoclonal antibody (M05), clone 4F5. Lane 1: KRT13 transfected lysate (Predicted MW: 49.6 KDa). Lane 2: Non-transfected lysate.
Sandwich ELISA: Cytokeratin 13 Antibody (4F5) [H00003860-M05] - Detection limit for recombinant GST tagged KRT13 is 1 ng/ml as a capture antibody.

Applications for Cytokeratin 13 Antibody (4F5)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Cytokeratin 13

The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in this gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus. The type I cytokeratins are clustered in a region of chromosome 17q21.2. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been described. [provided by RefSeq]

Alternate Names

CK13, CK-13, cytokeratin 13, Cytokeratin-13, K13cytokeratin-13, keratin 13, keratin, type I cytoskeletal 13, keratin-13, MGC161462, MGC3781

Entrez Gene IDs

3860 (Human)

Gene Symbol

KRT13

UniProt

NP_705694 (Human)

Additional Cytokeratin 13 Products

Product Documents for Cytokeratin 13 Antibody (4F5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Cytokeratin 13 Antibody (4F5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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