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Cytokeratin 13 Antibody (KRT13/2659)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07847

Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-07847-20ug
NBP3-07847-100ug

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # KRT13/2659

Concentration

0.2 mg/ml

Product Specifications

Immunogen

Recombinant full-length human Cytokeratin 13 protein (Uniprot: P13646)

Localization

Cytoplasmic

Marker

Non-Keratinized Squamous Epithelial Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08284)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Cytokeratin 13 Antibody (KRT13/2659)

Immunohistochemistry-Paraffin: Cytokeratin 13 Antibody (KRT13/2659) [NBP3-07847]

Immunohistochemistry-Paraffin: Cytokeratin 13 Antibody (KRT13/2659) [NBP3-07847]

Immunohistochemistry-Paraffin: Cytokeratin 13 Antibody (KRT13/2659) [NBP3-07847] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 13 Mouse Monoclonal Antibody (KRT13/2659).
Flow Cytometry: Cytokeratin 13 Antibody (KRT13/2659) [NBP3-07847]

Flow Cytometry: Cytokeratin 13 Antibody (KRT13/2659) [NBP3-07847]

Flow Cytometry: Cytokeratin 13 Antibody (KRT13/2659) [NBP3-07847] - Flow Cytometric Analysis of HeLa cells using Cytokeratin 13 Mouse Monoclonal Antibody (KRT13/2659) followed by goat anti- Mouse IgG-CF488 (Blue); Isotype Control (Red).
Immunohistochemistry-Paraffin: Cytokeratin 13 Antibody (KRT13/2659) [NBP3-07847]

Immunohistochemistry-Paraffin: Cytokeratin 13 Antibody (KRT13/2659) [NBP3-07847]

Immunohistochemistry-Paraffin: Cytokeratin 13 Antibody (KRT13/2659) [NBP3-07847] - Formalin-fixed, paraffin-embedded human Tonsil stained with Cytokeratin 13 Mouse Monoclonal Antibody (KRT13/2659).

Applications for Cytokeratin 13 Antibody (KRT13/2659)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cytokeratin 13

Alternate Names

CK13, CK-13, cytokeratin 13, Cytokeratin-13, K13cytokeratin-13, keratin 13, keratin, type I cytoskeletal 13, keratin-13, MGC161462, MGC3781

Gene Symbol

KRT13

UniProt

Additional Cytokeratin 13 Products

Product Documents for Cytokeratin 13 Antibody (KRT13/2659)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Cytokeratin 13 Antibody (KRT13/2659)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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