Cytokeratin 13 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-06047

Novus Biologicals, part of Bio-Techne
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NBP1-06047
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Cytokeratin 13 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-TSNASGRRTSDVRRP corresponding to C-Terminus according to NP_705694.2.

Epitope

C-TSNASGRRTSDVRRP

Specificity

This antibody is expected to recognize the reported isoform a (NP_002265.2) only.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Cytokeratin 13 Antibody

Western Blot: Cytokeratin 13 Antibody [NBP1-06047]

Western Blot: Cytokeratin 13 Antibody [NBP1-06047]

Western Blot: Cytokeratin 13 Antibody [NBP1-06047] - (0.1ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Cytokeratin 13 Antibody [NBP1-06047]

Immunohistochemistry-Paraffin: Cytokeratin 13 Antibody [NBP1-06047]

Immunohistochemistry-Paraffin: Cytokeratin 13 Antibody [NBP1-06047] - (2.5ug/ml) staining Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Cytokeratin 13 Antibody

Application
Recommended Usage

Immunohistochemistry

2 - 4 ug/ml

Immunohistochemistry-Paraffin

2 - 4 ug/ml

Peptide ELISA

Detection limit 1:128000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 55 kDa band observed in human lung, skeletal muscle and skin lysates is reported in scientific literature (PMID: 12686638). IHC-P: Human tonsil shows strong layered, filamentous staining.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cytokeratin 13

The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in this gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus. The type I cytokeratins are clustered in a region of chromosome 17q21.2. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been described. [provided by RefSeq]

Alternate Names

CK13, CK-13, cytokeratin 13, Cytokeratin-13, K13cytokeratin-13, keratin 13, keratin, type I cytoskeletal 13, keratin-13, MGC161462, MGC3781

Entrez Gene IDs

3860 (Human)

Gene Symbol

KRT13

UniProt

NP_705694.2 (Human)

Additional Cytokeratin 13 Products

Product Documents for Cytokeratin 13 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Cytokeratin 13 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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