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Cytokeratin 15 Antibody (LHK15) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-50461

Novus Biologicals, part of Bio-Techne
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NBP2-50461

Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # LHK15

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Peptide sequence of Cytokeratin 15 - BSA conjugate

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Cytokeratin 15 Antibody (LHK15) - BSA Free

Flow Cytometry: Cytokeratin 15 Antibody (LHK15) - BSA Free [NBP2-50461]

Flow Cytometry: Cytokeratin 15 Antibody (LHK15) - BSA Free [NBP2-50461]

Flow Cytometry: Cytokeratin 15 Antibody (LHK 15) [NBP2-50461] - An intracellular stain was performed on HeLa cells with Cytokeratin 15 Antibody (LHK 15) NBP2-50461PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.

Applications for Cytokeratin 15 Antibody (LHK15) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Flow Cytometry

1:10 - 1:1000

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:10 - 1:500

Immunoprecipitation

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
Positive control(s): Mouse colon tissue (IHC), A431 cells (IF), HepG2 cells (IF), COS7 cells (IF). This antibody is Cytof ready.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytokeratin 15

Alternate Names

CK15, CK-15, cytokeratin 15, cytokeratin-15, K15keratin-15, basic, K1CO, keratin 15, keratin, type I cytoskeletal 15, Keratin-15, keratin-15, beta, KRTB, type I cytoskeletal 15

Gene Symbol

KRT15

Additional Cytokeratin 15 Products

Product Documents for Cytokeratin 15 Antibody (LHK15) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Cytokeratin 15 Antibody (LHK15) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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