Cytokeratin 16 Antibody (8L6R4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16678

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16678-100ul
NBP3-16678-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8L6R4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Cytokeratin 16 Antibodies »

Product Summary for Cytokeratin 16 Antibody (8L6R4)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Cytokeratin 16 (P08779). MTTCSRQFTSSSSMKGSCGIGGGIGGGSSRISSVLAGGSCRAPSTYGGGLSVSSRFSSGGACGLGGGYGGGFSSSSSFGSGFGGGYGGGLGAGFGGGLGA

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cytokeratin 16 Antibody (8L6R4)

Western Blot: Cytokeratin 16 Antibody (8L6R4) [NBP3-16678]

Western Blot: Cytokeratin 16 Antibody (8L6R4) [NBP3-16678]

Western Blot: Cytokeratin 16 Antibody (8L6R4) [NBP3-16678] - Western blot analysis of extracts of Mouse large intestine cells, using Cytokeratin 16 antibody (NBP3-16678) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
Immunohistochemistry: Cytokeratin 16 Antibody (8L6R4) [NBP3-16678]

Immunohistochemistry: Cytokeratin 16 Antibody (8L6R4) [NBP3-16678]

Immunohistochemistry: Cytokeratin 16 Antibody (8L6R4) [NBP3-16678] - Immunofluorescence analysis of Mouse tail and ear using Cytokeratin 16 Rabbit mAb (NBP3-16678) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: Cytokeratin 16 Antibody (8L6R4) [NBP3-16678]

Immunohistochemistry-Paraffin: Cytokeratin 16 Antibody (8L6R4) [NBP3-16678]

Immunohistochemistry-Paraffin: Cytokeratin 16 Antibody (8L6R4) [NBP3-16678] - Immunohistochemistry of paraffin-embedded human esophageal cancer using Cytokeratin 16 Rabbit mAb (NBP3-16678) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Applications for Cytokeratin 16 Antibody (8L6R4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cytokeratin 16

Alternate Names

CK16, CK-16, cytokeratin 16, Cytokeratin-16, FNEPPK, focal non-epidermolytic palmoplantar keratoderma, K16, K1CP, keratin 16, keratin, type I cytoskeletal 16, keratin-16, KRT16A, NEPPK

Gene Symbol

KRT16

Additional Cytokeratin 16 Products

Product Documents for Cytokeratin 16 Antibody (8L6R4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cytokeratin 16 Antibody (8L6R4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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