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Cytokeratin 16 Antibody (KRT16/1714)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07648

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # KRT16/1714

Concentration

0.2 mg/ml

Product Specifications

Immunogen

Recombinant fragment from the C-terminal of human Cytokeratin 16 (Uniprot: P08779)

Localization

Cytoplasmic

Marker

Suprabasal Keratinocyte Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08691)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Cytokeratin 16 Antibody (KRT16/1714)

Immunohistochemistry-Paraffin: Cytokeratin 16 Antibody (KRT16/1714) [NBP3-07648]

Immunohistochemistry-Paraffin: Cytokeratin 16 Antibody (KRT16/1714) [NBP3-07648]

Immunohistochemistry-Paraffin: Cytokeratin 16 Antibody (KRT16/1714) [NBP3-07648] - Formalin-fixed, paraffin-embedded human Tonsil stained with Cytokeratin 16 Mouse Monoclonal Antibody (KRT16/1714).

Applications for Cytokeratin 16 Antibody (KRT16/1714)

Application
Recommended Usage

Immunohistochemistry-Paraffin

0.1-0.2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.1-0.2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cytokeratin 16

Alternate Names

CK16, CK-16, cytokeratin 16, Cytokeratin-16, FNEPPK, focal non-epidermolytic palmoplantar keratoderma, K16, K1CP, keratin 16, keratin, type I cytoskeletal 16, keratin-16, KRT16A, NEPPK

Gene Symbol

KRT16

UniProt

Additional Cytokeratin 16 Products

Product Documents for Cytokeratin 16 Antibody (KRT16/1714)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Cytokeratin 16 Antibody (KRT16/1714)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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