Cytokeratin 16 Antibody (SC52-09)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67559
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # SC52-09
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within huamn Cytokeratin 16 aa 27-75 / 473. (SwissProt: P08779 Human)
Localization
Cytoskeleton, intermediate filament, cytosol, extracellular exosome, nucleus.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Cytokeratin 16 Antibody (SC52-09)
Western Blot: Cytokeratin 16 Antibody (SC52-09) [NBP2-67559]
Western Blot: Cytokeratin 16 Antibody (SC52-09) [NBP2-67559] - Analysis of Cytokeratin 16 on human skin lysates using anti-Cytokeratin 16 antibody at 1/1,000 dilution.Immunocytochemistry/ Immunofluorescence: Cytokeratin 16 Antibody (SC52-09) [NBP2-67559]
Immunocytochemistry/Immunofluorescence: Cytokeratin 16 Antibody (SC52-09) [NBP2-67559] - Staining Cytokeratin 16 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Immunohistochemistry-Paraffin: Cytokeratin 16 Antibody (SC52-09) [NBP2-67559]
Immunohistochemistry-Paraffin: Cytokeratin 16 Antibody (SC52-09) [NBP2-67559] - Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Cytokeratin 16 with Rabbit anti-Cytokeratin 16 antibody washed with PBS, and then probed with the primary antibody (red) at 1/200 dilution overnight at 4 , washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).Applications for Cytokeratin 16 Antibody (SC52-09)
Application
Recommended Usage
Flow Cytometry
1:50-1:100
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Cytokeratin 16
Alternate Names
CK16, CK-16, cytokeratin 16, Cytokeratin-16, FNEPPK, focal non-epidermolytic palmoplantar keratoderma, K16, K1CP, keratin 16, keratin, type I cytoskeletal 16, keratin-16, KRT16A, NEPPK
Gene Symbol
KRT16
Additional Cytokeratin 16 Products
Product Documents for Cytokeratin 16 Antibody (SC52-09)
Product Specific Notices for Cytokeratin 16 Antibody (SC52-09)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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