Cytokeratin 20 Antibody (A3H7-R)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32249

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-32249
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # A3H7-R

Concentration

1 mg/ml

View all Cytokeratin 20 Antibodies »

Product Summary for Cytokeratin 20 Antibody (A3H7-R)

Immunogen

Synthetic peptide within C-terminal Human Cytokeratin 20. (Uniprot: P35900)

Localization

Cytoplasm.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cytokeratin 20 Antibody (A3H7-R)

Cytokeratin 20 Antibody (A3H7-R)

Immunohistochemistry: Cytokeratin 20 Antibody (A3H7-R) [NBP3-32249]

Immunohistochemistry: Cytokeratin 20 Antibody (A3H7-R) [NBP3-32249] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Mouse anti-Cytokeratin 20 antibody (NBP3-32249) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32249) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Applications for Cytokeratin 20 Antibody (A3H7-R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytokeratin 20

Intermediate-sized filament (IF) protein designated cytokeratin (CK) 1 is a major cellular protein of mature enterocytes and goblet cells commonly found in mucosal epithelium of the mammalian gastrointestinal tract (1). Results strongly suggest that transcriptional regulation of keratin genes in the intestinal epithelium occurs at the level of both immature and terminally differentiated epithelial cells, and is tightly regulated during both fetal development and crypt-to-villus differentiation of the intestinal epithelium (2). CK20 has recently been reported to be useful to distinguish between primary and metastatic lung adenocarcinoma. CK20 expression was significantly more prevalent in adenocarcinoma that originated in the GI tract than that of pulmonary or breast origin (3).

Alternate Names

CD20, CK20, CK-20, Cytokeratin-20, K20cytokeratin 20, keratin 20, keratin, type I cytoskeletal 20, keratin-20, KRT21, MGC35423, Protein IT

Gene Symbol

KRT20

Additional Cytokeratin 20 Products

Product Documents for Cytokeratin 20 Antibody (A3H7-R)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cytokeratin 20 Antibody (A3H7-R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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