Cytokeratin 20 Antibody (OTI4A2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45782

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4A2

Concentration

1 mg/ml

View all Cytokeratin 20 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-266 of human KRT20 (NP_061883) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

48.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cytokeratin 20 Antibody (OTI4A2)

Western Blot: Cytokeratin 20 Antibody (OTI4A2) [NBP2-45782]

Western Blot: Cytokeratin 20 Antibody (OTI4A2) [NBP2-45782]

Western Blot: Cytokeratin 20 Antibody (4A2) [NBP2-45782] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytokeratin 20.
Immunohistochemistry: Cytokeratin 20 Antibody (OTI4A2) [NBP2-45782]

Immunohistochemistry: Cytokeratin 20 Antibody (OTI4A2) [NBP2-45782]

Immunohistochemistry: Cytokeratin 20 Antibody (4A2) [NBP2-45782] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: Cytokeratin 20 Antibody (OTI4A2) [NBP2-45782]

Immunohistochemistry: Cytokeratin 20 Antibody (OTI4A2) [NBP2-45782]

Immunohistochemistry: Cytokeratin 20 Antibody (4A2) [NBP2-45782] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Applications for Cytokeratin 20 Antibody (OTI4A2)

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cytokeratin 20

Intermediate-sized filament (IF) protein designated cytokeratin (CK) 1 is a major cellular protein of mature enterocytes and goblet cells commonly found in mucosal epithelium of the mammalian gastrointestinal tract (1). Results strongly suggest that transcriptional regulation of keratin genes in the intestinal epithelium occurs at the level of both immature and terminally differentiated epithelial cells, and is tightly regulated during both fetal development and crypt-to-villus differentiation of the intestinal epithelium (2). CK20 has recently been reported to be useful to distinguish between primary and metastatic lung adenocarcinoma. CK20 expression was significantly more prevalent in adenocarcinoma that originated in the GI tract than that of pulmonary or breast origin (3).

Alternate Names

CD20, CK20, CK-20, Cytokeratin-20, K20cytokeratin 20, keratin 20, keratin, type I cytoskeletal 20, keratin-20, KRT21, MGC35423, Protein IT

Gene Symbol

KRT20

Additional Cytokeratin 20 Products

Product Documents for Cytokeratin 20 Antibody (OTI4A2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Cytokeratin 20 Antibody (OTI4A2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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