Cytokeratin 3 Antibody (KRT3/2130)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-79718

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # KRT3/2130

Concentration

0.2 mg/ml

View all Cytokeratin 3 Antibodies »

Product Specifications

Immunogen

Recombinant full-length human Cytokeratin 3 protein (Uniprot: P12035)

Localization

Cytoplasmic

Marker

Corneal Epithelial Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79852)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Cytokeratin 3 Antibody (KRT3/2130)

Immunohistochemistry-Paraffin: Cytokeratin 3 Antibody (KRT3/2130) [NBP2-79718]

Immunohistochemistry-Paraffin: Cytokeratin 3 Antibody (KRT3/2130) [NBP2-79718]

Immunohistochemistry-Paraffin: Cytokeratin 3 Antibody (KRT3/2130) [NBP2-79718] - Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin 3 Antibody (KRT3/2130).
Immunohistochemistry-Paraffin: Cytokeratin 3 Antibody (KRT3/2130) [NBP2-79718]

Immunohistochemistry-Paraffin: Cytokeratin 3 Antibody (KRT3/2130) [NBP2-79718]

Immunohistochemistry-Paraffin: Cytokeratin 3 Antibody (KRT3/2130) [NBP2-79718] - Formalin-fixed, paraffin-embedded human Ovary stained with Cytokeratin 3 Antibody (KRT3/2130).
Protein Array: Cytokeratin 3 Antibody (KRT3/2130) [NBP2-79718]

Protein Array: Cytokeratin 3 Antibody (KRT3/2130) [NBP2-79718]

Protein Array: Cytokeratin 3 Antibody (KRT3/2130) [NBP2-79718] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Cytokeratin 3 Antibody (KRT3/2130). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

Applications for Cytokeratin 3 Antibody (KRT3/2130)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1:2000
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cytokeratin 3

The protein encoded by the Cytokeratin 3 gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the corneal epithelium with family member KRT12 and mutations in these genes have been associated with Meesmann's Corneal Dystrophy. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. (provided by RefSeq)

Alternate Names

CK3, CK-3, cytokeratin-3, FLJ95909, K3cytokeratin 3, keratin 3, keratin, type II cytoskeletal 3,65 kDa cytokeratin, Keratin-3, Type-II keratin Kb3

Gene Symbol

KRT3

UniProt

P12035 (Human)

Additional Cytokeratin 3 Products

Product Documents for Cytokeratin 3 Antibody (KRT3/2130)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Cytokeratin 3 Antibody (KRT3/2130)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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