Cytokeratin 4 Antibody (5H5)

Novus Biologicals, part of Bio-Techne | Catalog # H00003851-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 5H5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Cytokeratin 4 Antibodies »

Product Specifications

Immunogen

KRT4 (NP_002263, 194 a.a. ~ 300 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SSKNLEPLFETYLSVLRKQLDTLGNDKGRLQSELKTMQDSVEDFKTKYEEEINKRTAAENDFVVLKKDVDAAYLNKVELEAKVDSLNDEINFLKVLYDAELSQMQTH

Specificity

KRT4 - keratin 4

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Cytokeratin 4 Antibody (5H5)

Western Blot: Cytokeratin 4 Antibody (5H5) [H00003851-M01]

Western Blot: Cytokeratin 4 Antibody (5H5) [H00003851-M01]

Western Blot: Cytokeratin 4 Antibody (5H5) [H00003851-M01] - KRT4 monoclonal antibody (M01), clone 5H5 Analysis of KRT4 expression in A-431.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 4 Antibody (5H5) [H00003851-M01]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 4 Antibody (5H5) [H00003851-M01]

Immunocytochemistry/Immunofluorescence: Cytokeratin 4 Antibody (5H5) [H00003851-M01] - Analysis of monoclonal antibody to KRT4 on A-431 cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: Cytokeratin 4 Antibody (5H5) [H00003851-M01]

Immunohistochemistry-Paraffin: Cytokeratin 4 Antibody (5H5) [H00003851-M01]

Immunohistochemistry-Paraffin: Cytokeratin 4 Antibody (5H5) [H00003851-M01] - Analysis of monoclonal antibody to KRT4 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.
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Applications for Cytokeratin 4 Antibody (5H5)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Cytokeratin 4

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in differentiated layers of the mucosal and esophageal epithelia with family member KRT13. Mutations in these genes have been associated with White Sponge Nevus, characterized by oral, esophageal, and anal leukoplakia. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

Alternate Names

CK4, CK-4, CYK4cytokeratin-4, Cytokeratin-4, FLJ31692, K4cytokeratin 4, keratin 4, keratin, type II cytoskeletal 4, Keratin-4, Type-II keratin Kb4

Entrez Gene IDs

3851 (Human)

Gene Symbol

KRT4

OMIM

123940 (Human)

UniProt

NP_002263 (Human)

Additional Cytokeratin 4 Products

Product Documents for Cytokeratin 4 Antibody (5H5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cytokeratin 4 Antibody (5H5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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