Cytokeratin 4 Antibody (KRT4/2804)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07797

Novus Biologicals, part of Bio-Techne
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NBP3-07797-20ug
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # KRT4/2804

Concentration

0.2 mg/ml

View all Cytokeratin 4 Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa 181-292) of human Cytokeratin 4 protein (exact sequence is proprietary) (Uniprot: P19013)

Localization

Cytoplasmic and Cell Surface

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08583)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Cytokeratin 4 Antibody (KRT4/2804)

Immunocytochemistry/ Immunofluorescence: Cytokeratin 4 Antibody (KRT4/2804) [NBP3-07797]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 4 Antibody (KRT4/2804) [NBP3-07797]

Immunocytochemistry/Immunofluorescence: Cytokeratin 4 Antibody (KRT4/2804) [NBP3-07797] - Immunofluorescence Analysis of A549 cells labeling KRT4 with Cytokeratin 4 Mouse Monoclonal Antibody (KRT4/2804)followed by goat anti- Mouse IgG-CF488 (Green). The nuclear counterstain is RedDot (Red).
Immunohistochemistry-Paraffin: Cytokeratin 4 Antibody (KRT4/2804) [NBP3-07797]

Immunohistochemistry-Paraffin: Cytokeratin 4 Antibody (KRT4/2804) [NBP3-07797]

Immunohistochemistry-Paraffin: Cytokeratin 4 Antibody (KRT4/2804) [NBP3-07797] - Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin 4 Mouse Monoclonal Antibody (KRT4/2804).
Flow Cytometry: Cytokeratin 4 Antibody (KRT4/2804) [NBP3-07797]

Flow Cytometry: Cytokeratin 4 Antibody (KRT4/2804) [NBP3-07797]

Flow Cytometry: Cytokeratin 4 Antibody (KRT4/2804) [NBP3-07797] - Flow Cytometric Analysis of HeLa cells using Cytokeratin 4 Mouse Monoclonal Antibody (KRT4/2804)followed by goat anti- Mouse IgG-CF488 (Blue); Isotype Control (Red).
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Applications for Cytokeratin 4 Antibody (KRT4/2804)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cytokeratin 4

Keratins are a family of structurally related proteins that form the intermediate filament cytoskeleton in epithelial cells. White sponge nevus (WSN) is a benign autosomal dominant disorder which affects non-cornifying stratified squamous epithelial. Keratins are expressed in pairs by epithelial cells in a tissue and cell specific manner. The major differentiation specific keratins of the buccal mucosa, nasal, esophageal and anogenital epithelia are CK-4 and CK-13. The tissue distribution and nature of the lesions in patients affected by WSN suggested that mutations in CK-4 and/or CK-13 might be responsible for this disorder (1). K4 and K13 form heterodimers in normal epithelial cells. In humans, K4 is present in all layers of the epidermis at 10 weeks and gradually disappears, starting from the basal layers at 15 weeks and becoming totally absent at around 20 weeks. This period corresponds to the early fetal period when melanoblasts derived from the neural crest migrate through the dermis into the basal layer of the epidermis (2)

Alternate Names

CK4, CK-4, CYK4cytokeratin-4, Cytokeratin-4, FLJ31692, K4cytokeratin 4, keratin 4, keratin, type II cytoskeletal 4, Keratin-4, Type-II keratin Kb4

Gene Symbol

KRT4

UniProt

P19013 (Human)

Additional Cytokeratin 4 Products

Product Documents for Cytokeratin 4 Antibody (KRT4/2804)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cytokeratin 4 Antibody (KRT4/2804)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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