Cytokeratin 5/6 Antibody (A2A12)

Western Blot: Cytokeratin 5/6 Antibody (A2A12) [NBP2-80401]

Western Blot: Cytokeratin 5/6 Antibody (A2A12) [NBP2-80401] - Analysis of Cytokeratin 6a on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (NBP2-80401, 1/100) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: HT-29 cell lysateLane 2: Human skin tissue lysate

Immunohistochemistry-Paraffin: Cytokeratin 5/6 Antibody (A2A12) [NBP2-80401]

Immunohistochemistry-Paraffin: Cytokeratin 5/6 Antibody (A2A12) [NBP2-80401] - Analysis in human esophagus tissue using Cytokeratin 6a antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, w

Flow Cytometry: Cytokeratin 5/6 Antibody (A2A12) [NBP2-80401]

Flow Cytometry: Cytokeratin 5/6 Antibody (A2A12) [NBP2-80401] - Analysis of Cytokeratin 6a was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).