Cytokeratin 5/75 Antibody (SC62-04)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67586

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-67586
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SC62-04

Concentration

1 mg/ml

View all Cytokeratin 5/75 Antibodies »

Product Specifications

Immunogen

Synthetic peptide within human Cytokeratin 5/75 aa 70-110. (SwissProt: P13647 Human)

Localization

Cytoplasm.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cytokeratin 5/75 Antibody (SC62-04)

Western Blot: Cytokeratin 5/75 Antibody (SC62-04) [NBP2-67586]

Western Blot: Cytokeratin 5/75 Antibody (SC62-04) [NBP2-67586]

Western Blot: Cytokeratin 5/75 Antibody (SC62-04) [NBP2-67586] - Western blot analysis of Cytokeratin 5/75 on A431 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Go
Immunohistochemistry-Paraffin: Cytokeratin 5/75 Antibody (SC62-04) [NBP2-67586]

Immunohistochemistry-Paraffin: Cytokeratin 5/75 Antibody (SC62-04) [NBP2-67586]

Immunohistochemistry-Paraffin: Cytokeratin 5/75 Antibody (SC62-04) [NBP2-67586] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 5/75 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-43, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western Blot: Cytokeratin 5/75 Antibody (SC62-04) [NBP2-67586]

Western Blot: Cytokeratin 5/75 Antibody (SC62-04) [NBP2-67586]

Western Blot: Cytokeratin 5/75 Antibody (SC62-04) [NBP2-67586] - Analysis of Cytokeratin 5 on A431 cell lysates using anti-Cytokeratin 5 antibody at 1/1,000 dilution.
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Applications for Cytokeratin 5/75 Antibody (SC62-04)

Application
Recommended Usage

Flow Cytometry

1:1000

Immunocytochemistry/ Immunofluorescence

1:200

Immunohistochemistry-Paraffin

1:50-1:500

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytokeratin 5/75

Alternate Names

58 kDa cytokeratin, CK5, CK-5, Cytokeratin-5, DDD, EBS2, epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types, K5, keratin 5, keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockaynetypes), keratin, type II cytoskeletal 5, Keratin-5, KRT5A, Type-II keratin Kb5

Gene Symbol

KRT5

Additional Cytokeratin 5/75 Products

Product Documents for Cytokeratin 5/75 Antibody (SC62-04)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cytokeratin 5/75 Antibody (SC62-04)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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