Cytokeratin 5/8 Antibody (C-50) [Janelia Fluor® 525]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47824JF525
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken (Negative), Ferret, Hamster, Sheep, Xenopus (Negative)
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Janelia Fluor 525
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # C-50
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Cytoskeletal preparation from HeLa cells
Reactivity Notes
Does not react with Chicken or Xenopus.
Localization
Cytoplasmic
Specificity
It reacts with keratin 5 (58kDa) and keratin 8 (52.5kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Applications for Cytokeratin 5/8 Antibody (C-50) [Janelia Fluor® 525]
Application
Recommended Usage
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Cytokeratin 5/8
Alternate Names
cell proliferation-inducing protein 46, CK18, CK5, CYK18, DDD, K18, K1CO, K5, Keratin, Keratin 18, Keratin 5, KRT18, krt5, KRT5A, KRTB, type I cytoskeletal 18, type II cytoskeletal 5
Gene Symbol
KRT5
Additional Cytokeratin 5/8 Products
Product Documents for Cytokeratin 5/8 Antibody (C-50) [Janelia Fluor® 525]
Product Specific Notices for Cytokeratin 5/8 Antibody (C-50) [Janelia Fluor® 525]
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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