Cytokeratin 5 Antibody (CL9297)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-88923

Novus Biologicals, part of Bio-Techne
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NBP2-88923
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL9297

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Cytokeratin 5 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a synthetic peptide corresponding to amino acids: SFRSGGSRSFSTASAITPSVSRTS

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Cytokeratin 5 Antibody (CL9297)

Western Blot: Cytokeratin 5 Antibody (CL9297) [NBP2-88923]

Western Blot: Cytokeratin 5 Antibody (CL9297) [NBP2-88923]

Western Blot: Cytokeratin 5 Antibody (CL9297) [NBP2-88923] - Analysis in human skin tissue.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 5 Antibody (CL9297) [NBP2-88923]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 5 Antibody (CL9297) [NBP2-88923]

Immunocytochemistry/Immunofluorescence: Cytokeratin 5 Antibody (CL9297) [NBP2-88923] - Staining of A-431 cells using the Anti-KRT5 monoclonal antibody, showing specific staining in intermediate filaments in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (CL9297) [NBP2-88923]

Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (CL9297) [NBP2-88923]

Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (CL9297) [NBP2-88923] - Analysis in human prostate and kidney tissues using NBP2-88923 antibody. Corresponding KRT5 RNA-seq data are presented for the same tissues.
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Applications for Cytokeratin 5 Antibody (CL9297)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, containing 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytokeratin 5

Keratins are a family of structurally related proteins that form the intermediate filament cytoskeleton in epithelial cells. The 58-kD keratin CK-5 is highly similar to other type II keratins and less similar to type I keratins and other intermediate filament proteins. The 58-kD keratin is regulated by retinoids in several tissues and is one of four keratins abundantly expressed in epidermal keratinocytes, where it may be important in maintaining structural integrity of the integument (1). Keratin 5 (CK-5) mRNA and protein are shown to be expressed in normal mammary epithelial cells in culture and are absent from tumor-derived cell lines. This makes CK-5 an important marker in the tumorigenic process, distinguishing normal from tumor cells, and decreased CK-5 expression correlates with tumorigenic progression (2). Dowling-Degos disease (DDD) is an autosomal dominant genodermatosis characterized by progressive and disfiguring reticulate hyperpigmentation of the flexures. Loss of function of CK-5 suggests a crucial role for keratins in the organization of cell adhesion, melanosome uptake, organelle transport, and nuclear anchorage (3).

Alternate Names

CK-5, DDD, EBS2, Keratin 5, KRT5

Gene Symbol

KRT5

Additional Cytokeratin 5 Products

Product Documents for Cytokeratin 5 Antibody (CL9297)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Cytokeratin 5 Antibody (CL9297)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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