Cytokeratin 6a Antibody (KRT6/3997R)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13955

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # KRT6/3997R

Concentration

0.2 mg/ml

View all Cytokeratin 6a Antibodies »

Product Summary for Cytokeratin 6a Antibody (KRT6/3997R)

Immunogen

Recombinant fragment of human Cytokeratin 6a (exact sequence is proprietary) (Uniprot: P02538)

Localization

Cytoplasm

Marker

Basal Cell Marker

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14107)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Cytokeratin 6a Antibody (KRT6/3997R)

Immunohistochemistry-Paraffin: Cytokeratin 6a Antibody (KRT6/3997R) [NBP3-13955]

Immunohistochemistry-Paraffin: Cytokeratin 6a Antibody (KRT6/3997R) [NBP3-13955]

Immunohistochemistry-Paraffin: Cytokeratin 6a Antibody (KRT6/3997R) [NBP3-13955] - Formalin-fixed, paraffin-embedded human prostatestained with Cytokeratin 6a antibody (KRT6/3997R). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.
Immunohistochemistry-Paraffin: Cytokeratin 6a Antibody (KRT6/3997R) [NBP3-13955]

Immunohistochemistry-Paraffin: Cytokeratin 6a Antibody (KRT6/3997R) [NBP3-13955]

Immunohistochemistry-Paraffin: Cytokeratin 6a Antibody (KRT6/3997R) [NBP3-13955] - IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using Cytokeratin 6a antibody (KRT6/3997R)at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.
Cytokeratin 6a Antibody (KRT6/3997R)

Cytokeratin 6a Antibody (KRT6/3997R)

Formalin-fixed, paraffin-embedded human bladder carcinoma pT24 stained with Cytokeratin 6a antibody (KRT6/3997R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
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Applications for Cytokeratin 6a Antibody (KRT6/3997R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cytokeratin 6a

The human type II Cytokeratin 6 (CK6; 56 kDa) is expressed in a heterogeneous array of epithelial tissues under normal conditions, but is better known for its strong induction in stratified epithelia that feature an enhanced cell proliferation rate or abnormal differentiation. It has been demonstrated that CK6 isoform, CK6a, is clearly the dominant CK6 isoform in skin tissue samples and cultured epithelial cell lines and that the various isoforms are differentially regulated within and between epithelial tissue types (1). The murine genome is known to have two keratin 6 (CK6) genes, mouse CK6, CK6a and MK6b. These genes display a complex expression pattern with constitutive expression in the epithelia of oral mucosa, hair follicles, and nail beds (2). Pachyonychia congenital (PC) is characterized by hypertrophic nail dystrophy and associated ectodermal features. PC-1 subtype is associated with mutations in keratins 6a or 16, whereas PC-2 subtype is linked to mutations in keratins 6b or 17 (3).

Alternate Names

Allergen Hom s 5, CK6A, CK-6A, CK6C, CK6D, CK-6D, cytokeratin 6A, cytokeratin 6C, cytokeratin 6D, Cytokeratin-6A, Cytokeratin-6D, K6A, K6C, K6D, keratin 6A, keratin 6C, keratin 6D, keratin, epidermal type II, K6A, keratin, type II cytoskeletal 6A, Keratin-6A, KRT6C, KRT6D, Type-II keratin Kb6

Gene Symbol

KRT6A

UniProt

P02538 (Human)

Additional Cytokeratin 6a Products

Product Documents for Cytokeratin 6a Antibody (KRT6/3997R)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cytokeratin 6a Antibody (KRT6/3997R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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