Cytokeratin 7 Antibody (R17-S)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30152
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # R17-S
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Peptide derived from N-terminal sequence of human cytokeratin 7. Antibody recognizes the epitope between Ala22 - Ser38.
Epitope
Ala22 - Ser38
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 28832662).
Specificity
This antibody is specific for the N terminus o Cytokeratin 7
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody is immunoaffinity purified with immunogenic peptide as a ligand.
Scientific Data Images for Cytokeratin 7 Antibody (R17-S)
Immunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody (R17-S) [NBP1-30152]
Immunocytochemistry/Immunofluorescence: Cytokeratin 7 Antibody (R17-S) [NBP1-30152] - A549 cell segregation and proliferation inside the spheroids. Four- day-old heterospheroids (as indicated) were double-stained with anti-human cytokeratin 7 (stained A549 cells, red) and anti-mouse beta1 integrin (stained MEFs, green) antibodies. Size bars = 100 um. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0103173) licensed under a CC-BY license.Immunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody (R17-S) [NBP1-30152]
Immunocytochemistry/Immunofluorescence: Cytokeratin 7 Antibody (R17-S) [NBP1-30152] - Spheroid migration on collagen I monolayers and invasion in 3D collagen gels. Fluorescence immunostaining of the spheroids on coverslips after 24 h and migration distance of A549 from the heterospheroids. Spheroids were double stained with antibodies towards human Cytokeratin 7 (stained A549 cells, red) and mouse beta1 integrin (stained MEFs, green). Six of each type of spheroids were stained. The maximum migration distance (d) of A549 cells were measured on the photographs as indicated in the figure and calculated according to size bar. Size bars = 100 um. **** p<0.0001. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0103173) licensed under a CC-BY license.Immunohistochemistry: Cytokeratin 7 Antibody (R17-S) [NBP1-30152]
Immunohistochemistry: Cytokeratin 7 Antibody (R17-S) [NBP1-30152] - Analysis of immunofluorecence stained collagen type I fibrils in primary tumors. The amount of stroma versus tumor cells demonstrates significantly less cancer cells in the HBOT group compared to control. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0183254), licensed under a CC-BY license.Applications for Cytokeratin 7 Antibody (R17-S)
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:100-200
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 25076207).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
20mM Tris-HCl (pH 8.0) and 20mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Cytokeratin 7
Additional Cytokeratin 7 Products
Product Documents for Cytokeratin 7 Antibody (R17-S)
Product Specific Notices for Cytokeratin 7 Antibody (R17-S)
This antibody is immunoaffinity purified with immunogenic peptide as a ligand.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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