Skip to main content

Cytokeratin 7 Antibody (R17-S)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30152

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
NBP1-30152-0.1ml

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # R17-S

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Peptide derived from N-terminal sequence of human cytokeratin 7. Antibody recognizes the epitope between Ala22 - Ser38.

Epitope

Ala22 - Ser38

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28832662).

Specificity

This antibody is specific for the N terminus o Cytokeratin 7

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is immunoaffinity purified with immunogenic peptide as a ligand.

Scientific Data Images for Cytokeratin 7 Antibody (R17-S)

Immunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody (R17-S) [NBP1-30152]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody (R17-S) [NBP1-30152]

Immunocytochemistry/Immunofluorescence: Cytokeratin 7 Antibody (R17-S) [NBP1-30152] - A549 cell segregation and proliferation inside the spheroids. Four- day-old heterospheroids (as indicated) were double-stained with anti-human cytokeratin 7 (stained A549 cells, red) and anti-mouse beta1 integrin (stained MEFs, green) antibodies. Size bars = 100 um. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0103173) licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody (R17-S) [NBP1-30152]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody (R17-S) [NBP1-30152]

Immunocytochemistry/Immunofluorescence: Cytokeratin 7 Antibody (R17-S) [NBP1-30152] - Spheroid migration on collagen I monolayers and invasion in 3D collagen gels. Fluorescence immunostaining of the spheroids on coverslips after 24 h and migration distance of A549 from the heterospheroids. Spheroids were double stained with antibodies towards human Cytokeratin 7 (stained A549 cells, red) and mouse beta1 integrin (stained MEFs, green). Six of each type of spheroids were stained. The maximum migration distance (d) of A549 cells were measured on the photographs as indicated in the figure and calculated according to size bar. Size bars = 100 um. **** p<0.0001. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0103173) licensed under a CC-BY license.
Immunohistochemistry: Cytokeratin 7 Antibody (R17-S) [NBP1-30152]

Immunohistochemistry: Cytokeratin 7 Antibody (R17-S) [NBP1-30152]

Immunohistochemistry: Cytokeratin 7 Antibody (R17-S) [NBP1-30152] - Analysis of immunofluorecence stained collagen type I fibrils in primary tumors. The amount of stroma versus tumor cells demonstrates significantly less cancer cells in the HBOT group compared to control. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0183254), licensed under a CC-BY license.

Applications for Cytokeratin 7 Antibody (R17-S)

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:100-200
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 25076207).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

20mM Tris-HCl (pH 8.0) and 20mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Cytokeratin 7

Cytokeratin 7 is a 54kD intermediate filament protein found in a variety of glandularepithelia. Cytokeratin 7 has been found in columnar and glandular epithelium of the lung, cervix, breast, bile ducts and larger collecting ducts of the kidney. It is present in the transitional epithelium of bladder as well as ovarian and lungepithelia, and occasionally staining of blood vessel cell walls, particularly endothelial cells, may be observed. However, Cytokeratin 7 is not expressed by epithelial cells of the gastrointestinal tract, colon or prostate. Keratin 7 is often co expressed with keratin 19.

Alternate Names

CK-7, CK7, K2C7, K7

Entrez Gene IDs

3855 (Human)

Gene Symbol

KRT7

UniProt

Additional Cytokeratin 7 Products

Product Documents for Cytokeratin 7 Antibody (R17-S)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Cytokeratin 7 Antibody (R17-S)

This antibody is immunoaffinity purified with immunogenic peptide as a ligand.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...