Cytokeratin 7 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33104

Novus Biologicals, part of Bio-Techne
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NBP1-33104
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation, Biological Validation

Species Reactivity

Validated:

Human

Predicted:

Chimpanzee (98%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Cytokeratin 7 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytokeratin 7. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (88%), Rhesus Macaque, Bovine (89%).

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cytokeratin 7 Antibody

Knockout Validated: Cytokeratin 7 Antibody [NBP1-33104]

Knockout Validated: Cytokeratin 7 Antibody [NBP1-33104]

Knockout Validated: Cytokeratin 7 Antibody [NBP1-33104] - Wild-type (WT) and Cytokeratin 7 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 7 antibody [N1C2]. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody [NBP1-33104]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody [NBP1-33104]

Immunocytochemistry/Immunofluorescence: Cytokeratin 7 Antibody [NBP1-33104] - Analysis of paraformaldehyde-fixed HeLa, using Cytokeratin.
Immunohistochemistry: Cytokeratin 7 Antibody [NBP1-33104]

Immunohistochemistry: Cytokeratin 7 Antibody [NBP1-33104]

Immunohistochemistry: Cytokeratin 7 Antibody [NBP1-33104] - Immunohistochemical characterization of cytokeratin 7 in human small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC) specimens.

Sample: Paraffin-embedded human SCLC (upper panel) and NSCLC (lower panel).

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 15 mins. The section was then incubated with primary antibody overnight at 4C and detected using an HRP conjugated avidin-biotin-peroxidase Complex system. DAB was used as the chromogen and counterstained with haematoxylin.
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Applications for Cytokeratin 7 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:5000-1:20000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Cytokeratin 7

Cytokeratin 7 is a 54kD intermediate filament protein found in a variety of glandularepithelia. Cytokeratin 7 has been found in columnar and glandular epithelium of the lung, cervix, breast, bile ducts and larger collecting ducts of the kidney. It is present in the transitional epithelium of bladder as well as ovarian and lungepithelia, and occasionally staining of blood vessel cell walls, particularly endothelial cells, may be observed. However, Cytokeratin 7 is not expressed by epithelial cells of the gastrointestinal tract, colon or prostate. Keratin 7 is often co expressed with keratin 19.

Alternate Names

CK-7, CK7, K2C7, K7

Gene Symbol

KRT7

Additional Cytokeratin 7 Products

Product Documents for Cytokeratin 7 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Cytokeratin 7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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