Cytokeratin 8/18 Antibody (KRT8.18/4997R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20574
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # KRT8.18/4997R
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant full-length human CK8 and CK8 proteins
Localization
Cytoplasm
Specificity
This monoclonal antibody cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stainedImmunohistochemical staining with this monoclonal antibody is indistinguishable from that obtained with monoclonal antibody 5D3
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Description
Positive Controls: MCF-7 or A431 cells. Human skin, colon, lung or breast carcinoma.
Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Non-hazardous. No MSDS required.
Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Non-hazardous. No MSDS required.
Scientific Data Images for Cytokeratin 8/18 Antibody (KRT8.18/4997R)
Cytokeratin 8/18 Antibody (KRT8.18/4997R)
Formalin-fixed, paraffin-embedded human prostate carcinoma stained with Cytokeratin 8/18 antibody (KRT8.18/4997R).18/4997R). Inset: PBS instead of primary antibody; secondary only negative control.Applications for Cytokeratin 8/18 Antibody (KRT8.18/4997R)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Cytokeratin 8/18
Alternate Names
CARD2, CK8, CK-8, CYK8cytokeratin 8, Cytokeratin-8, K2C8, K8cytokeratin-8, keratin 8, keratin, type II cytoskeletal 8, keratin-8, KO, Type-II keratin Kb8
Gene Symbol
KRT8
Additional Cytokeratin 8/18 Products
Product Documents for Cytokeratin 8/18 Antibody (KRT8.18/4997R)
Product Specific Notices for Cytokeratin 8/18 Antibody (KRT8.18/4997R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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