Cytokeratin 8 Antibody (C-43) - IHC-Prediluted
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48169
![Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (C-43) - IHC-Prediluted [NBP2-48169]](https://resources.bio-techne.com/images/products/Cytokeratin-8-Antibody-C-43-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48169-img0002.jpg "Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (C-43) - IHC-Prediluted [NBP2-48169]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Porcine, Bovine, Chicken (Negative), Mouse (Negative), Rabbit, Rat (Negative), Sheep, Xenopus (Negative)
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # C-43
Format
IHC-Prediluted
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Cytokeratin 8 Antibody (C-43) - IHC-Prediluted
Immunogen
Cytoskeletal preparation from HeLa cells
Reactivity Notes
Does not react with Mouse, Rat, Chicken or Xenopus laevis.
Localization
Cytoplasmic
Specificity
Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). CK8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin 8 and 18. CK8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18 and CK19). Reportedly, anti-CK8 is useful for the differentiation of lobular ('ring-like, perinuclear') from ductal ('peripheral-predominant') carcinoma of the breast.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
52.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
Scientific Data Images for Cytokeratin 8 Antibody (C-43) - IHC-Prediluted
Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (C-43) - IHC-Prediluted [NBP2-48169]
Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (C-43) - IHC-Prediluted [NBP2-48169] - Human Colon carcinoma stained with Cytokeratin 8 Monoclonal Antibody (C-43).Applications for Cytokeratin 8 Antibody (C-43) - IHC-Prediluted
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Hu-chromosome location: 12q13.13 Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Format
IHC-Prediluted
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Cytokeratin 8
Alternate Names
CARD2, CK8, EndoA, KRT8
Gene Symbol
KRT8
Additional Cytokeratin 8 Products
Product Documents for Cytokeratin 8 Antibody (C-43) - IHC-Prediluted
Product Specific Notices for Cytokeratin 8 Antibody (C-43) - IHC-Prediluted
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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