Cytokeratin, Acidic (Type I or LMW) Antibody (SPM115)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34346
![Immunohistochemistry-Paraffin: Cytokeratin, Acidic (Type I or LMW) Antibody (SPM115) [NBP2-34346]](https://resources.bio-techne.com/images/products/Cytokeratin-Acidic-Type-I-or-LMW-Antibody-SPM115-Immunohistochemistry-Paraffin-NBP2-34346-img0001.jpg "Immunohistochemistry-Paraffin: Cytokeratin, Acidic (Type I or LMW) Antibody (SPM115) [NBP2-34346]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Bovine, Canine, Chicken, Monkey, Rabbit, Reptile
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM115
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Human epidermal keratin (Uniprot: Q7Z794)
Reactivity Notes
Turtle
Localization
Cytoplasmic
Specificity
This monoclonal antibody recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40kDa. monoclonal antibody SPM116 recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. SPM115/SPM116 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.
Marker
Epithelial Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34393)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Cytokeratin, Acidic (Type I or LMW) Antibody (SPM115)
Immunohistochemistry-Paraffin: Cytokeratin, Acidic (Type I or LMW) Antibody (SPM115) [NBP2-34346]
Immunohistochemistry-Paraffin: Cytokeratin, Acidic (Type I or LMW) Antibody (SPM115) [NBP2-34346] - Formalin-fixed paraffin-embedded human tonsil stained with cytokeratin, LMW Monoclonal antibody (SPM115)Applications for Cytokeratin, Acidic (Type I or LMW) Antibody (SPM115)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Cytokeratin, Acidic (Type I or LMW)
Alternate Names
CK10, CK14, CK15, CK16, CK19, cytokeratin 10, cytokeratin 14, cytokeratin 15, cytokeratin 16, cytokeratin 19
UniProt
Additional Cytokeratin, Acidic (Type I or LMW) Products
Product Documents for Cytokeratin, Acidic (Type I or LMW) Antibody (SPM115)
Product Specific Notices for Cytokeratin, Acidic (Type I or LMW) Antibody (SPM115)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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