Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # KRTL/1077

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all Cytokeratin, LMW Antibodies »

Product Specifications

Immunogen

Recombinant human Cytokeratin, LMW protein (Uniprot: Q7Z794)

Reactivity Notes

Shows broad species reactivity.

Localization

Cytoplasmic

Specificity

This monoclonal antibody recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Marker

Epithelial Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44373).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Cytokeratin, LMW Antibody (KRTL/1077) - Azide and BSA Free

Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (KRTL/1077) - Azide and BSA Free [NBP2-47798]

Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (KRTL/1077) - Azide and BSA Free [NBP2-47798]

Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (KRTL/1077) - Azide and BSA Free [NBP2-47798] - Human Skin stained with Cytokeratin, LMW Monoclonal Antibody (KRTL/1077).
Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (KRTL/1077) - Azide and BSA Free [NBP2-47798]

Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (KRTL/1077) - Azide and BSA Free [NBP2-47798]

Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (KRTL/1077) - Azide and BSA Free [NBP2-47798] - Rat Oviduct stained with Cytokeratin, HMW Monoclonal Antibody (KRTL/1077).
Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (KRTL/1077) - Azide and BSA Free [NBP2-47798]

Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (KRTL/1077) - Azide and BSA Free [NBP2-47798]

Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (KRTL/1077) - Azide and BSA Free [NBP2-47798] - Rat Stomach with Cytokeratin, HMW Monoclonal Antibody (KRTL/1077).

Applications for Cytokeratin, LMW Antibody (KRTL/1077) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 0.1-0.2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Cytokeratin, LMW

Alternate Names

Cytokeratin LMW, cytokeratin-1B, keratin 77, keratin, type II cytoskeletal 1b, KRT77, type-II keratin Kb39

Gene Symbol

KRT77

Additional Cytokeratin, LMW Products

Product Documents for Cytokeratin, LMW Antibody (KRTL/1077) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Cytokeratin, LMW Antibody (KRTL/1077) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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