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Cytosolic Sulfotransferase 1A1/SULT1A1 Antibody (OTI9B7) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71308

Novus Biologicals, part of Bio-Techne
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NBP2-71308

Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI9B7

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human SULT1A1 (NP_001046) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images

Western Blot: Cytosolic Sulfotransferase 1A1/SULT1A1 Antibody (OTI9B7)Azide and BSA Free [NBP2-71308]

Western Blot: Cytosolic Sulfotransferase 1A1/SULT1A1 Antibody (OTI9B7)Azide and BSA Free [NBP2-71308]

Western Blot: Cytosolic Sulfotransferase 1A1/SULT1A1 Antibody (OTI9B7) - Azide and BSA Free [NBP2-71308] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SULT1A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SULT1A1.
Immunocytochemistry/ Immunofluorescence: Cytosolic Sulfotransferase 1A1/SULT1A1 Antibody (OTI9B7) - Azide and BSA Free [NBP2-71308]

Immunocytochemistry/ Immunofluorescence: Cytosolic Sulfotransferase 1A1/SULT1A1 Antibody (OTI9B7) - Azide and BSA Free [NBP2-71308]

Immunocytochemistry/Immunofluorescence: Cytosolic Sulfotransferase 1A1/SULT1A1 Antibody (OTI9B7) - Azide and BSA Free [NBP2-71308] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SULT1A1.
Immunohistochemistry: Cytosolic Sulfotransferase 1A1/SULT1A1 Antibody (OTI9B7) - Azide and BSA Free [NBP2-71308]

Immunohistochemistry: Cytosolic Sulfotransferase 1A1/SULT1A1 Antibody (OTI9B7) - Azide and BSA Free [NBP2-71308]

Immunohistochemistry: Cytosolic Sulfotransferase 1A1/SULT1A1 Antibody (OTI9B7) - Azide and BSA Free [NBP2-71308] - Staining of paraffin-embedded Human bladder tissue using anti-SULT1A1 mouse monoclonal antibody.

Applications

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cytosolic Sulfotransferase 1A1/SULT1A1

Mammalian EST sulfurylates the hydroxyl group of estrogenic steroids by transferring the sulfate from a cosubstrate adenosine 3 prime-phosphate-5 prime-phosphosulfate. Sulfurylated steroids do not bind to the oestrogen receptor with high affinity and, therefore, are hormonally inactive. EST plays an important role in controlling the intracellular level of the receptor-active estrogens.

Alternate Names

P-PST, PST, ST1A3, STP1, SULT1A1, TSPST1

Gene Symbol

SULT1A1

Additional Cytosolic Sulfotransferase 1A1/SULT1A1 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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