Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 97A1015

Format

Azide Free

Concentration

1.0 mg/ml

View all D4-GDI/RhoGDI2 Antibodies »

Product Summary for D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) - Azide Free

Immunogen

A synthetic peptide corresponding to the region of the Fas-induced cleavage site of human D4-GDI/RhoGDI2 was used as the immunogen.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) - Azide Free

Western Blot: D4-GDI/RhoGDI2 Antibody (97A1015)(cleavage specific)Azide Free [NBP2-33248]

Western Blot: D4-GDI/RhoGDI2 Antibody (97A1015)(cleavage specific)Azide Free [NBP2-33248]

Western Blot: D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) - Azide Free [NBP2-33248] - Western blot analysis of D4GDI in Jurkat cells using NB100-56556 (IMG-396A) at 2 ug/ml and NB100-56725 (IMG-160A) at 0.1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. NB100-56556 (IMG-396A) detects both the full-length and cleaved forms of D4GDI. While (NB100-56725) IMG-160A specifically detects only the cleaved form of the protein. Image from the standard format of this antibody.
Immunohistochemistry-Paraffin: D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) - Azide Free [NBP2-33248]

Immunohistochemistry-Paraffin: D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) - Azide Free [NBP2-33248]

Immunohistochemistry-Paraffin: D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) - Azide Free [NBP2-33248] - Analysis of D4GD1-cv in formalin-fixed, paraffin-embedded human breast tumor tissue using an isotype control (top) and this antibody. Image from the standard format of this antibody.
Western Blot: D4-GDI/RhoGDI2 Antibody (97A1015)(cleavage specific)Azide Free [NBP2-33248]

Western Blot: D4-GDI/RhoGDI2 Antibody (97A1015)(cleavage specific)Azide Free [NBP2-33248]

Western Blot: D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) - Azide Free [NBP2-33248] - WB analysis: HL60 cells were treated for different time periods with 10 ng/ml of anti-Fas mAb (NB100-56725, Clone DX2). Harvested cells were lysed and resolved by SDS-PAGE and transferred onto membrane. D4-GDI/RhoGDI2 cleavage was detected. Image from the standard format of this antibody.

Applications for D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) - Azide Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1-2 ug/ml

Western Blot

2 ug/ml
Application Notes
In anti-Fas treated Jurkat, an approx. 23 kDa band should be observed.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS - BSA free

Format

Azide Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: D4-GDI/RhoGDI2

Alternate Names

ARHGDIB Rho GDP dissociation inhibitor (GDI) beta, D4, GDIA2, GDID4, LYGDI, Ly-GDI, RAP1GN1, RhoGDI2

Gene Symbol

ARHGDIB

Additional D4-GDI/RhoGDI2 Products

Product Documents for D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) - Azide Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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