DAAM1 Antibody (5D3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23272

Novus Biologicals, part of Bio-Techne
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NBP3-23272
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 5D3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all DAAM1 Antibodies »

Product Summary for DAAM1 Antibody (5D3)

Immunogen

Clone 5D3 was generated from a recombinant protein that included amino acid residues from the N-terminal region of human DAAM1. This sequence has less than 50% homology to the N-terminal region of DAAM2. [UniProt# Q9Y4D1]

Specificity

The antibody detects a 125 kDa protein corresponding to the apparent molecular mass of DAAM1 on SDS-PAGE immunoblots of human A431, K562, and mouse C2C12 cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

125 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Applications for DAAM1 Antibody (5D3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:200

Western Blot

1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: DAAM1

Functions of the cell cortex, including motility, adhesion, and cytokinesis, are mediated by the reorganization of the actin cytoskeleton and recent evidence suggests a role for the Formin homology (FH) proteins in these processes. The protein encoded by this gene contains FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. Wnt/Fz signaling activates the small GTPase Rho, a key regulator of cytoskeleton architecture, to control cell polarity and movement during development. Activation requires Dvl-Rho complex formation, an assembly mediated by this gene product, which is thought to function as a scaffolding protein. Evidence of alternative splicing has been observed for this gene but the full-length nature of these variants has not been determined.

Alternate Names

disheveled-associated activator of morphogenesis 1, dishevelled associated activator of morphogenesis 1, dishevelled-associated activator of morphogenesis 1, KIAA0666FLJ41657

Gene Symbol

DAAM1

Additional DAAM1 Products

Product Documents for DAAM1 Antibody (5D3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DAAM1 Antibody (5D3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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