DACH2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-00177

Novus Biologicals, part of Bio-Techne
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NBP1-00177
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Key Product Details

Species Reactivity

Validated:

Mouse

Predicted:

Human (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all DACH2 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-TRKQAVNSSRPGR corresponding to internal region according to NP_444511.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for DACH2 Antibody

Western Blot: DACH2 Antibody [NBP1-00177]

Western Blot: DACH2 Antibody [NBP1-00177]

Western Blot: DACH2 Antibody [NBP1-00177] - Analysis of DACH2 in NIH3T3 cell lysate (35ug protein in RIPA buffer) using NBP1-00177 at 0.3ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for DACH2 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:64000

Western Blot

0.03 - 0.1 ug/ml
Application Notes
WB: Approx. 65-70 kDa band observed in NIH3T3 lysates (calculated MW of 65.3 kDa band according to human NP_444511.1 and of 68.6 kDa band according to mouse NP_291083.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DACH2

This gene is one of two genes which encode a protein similar to the Drosophila protein dachshund, a transcription factor involved in cell fate determination in the eye, limb and genital disc of the fly. The encoded protein contains two characteristic dachshund domains: an N-terminal domain responsible for DNA binding and a C-terminal domain responsible for protein-protein interactions. This gene is located on the X chromosome and is subject to inactivation by DNA methylation. The encoded protein may be involved in regulation of organogenesis and myogenesis, and may play a role in premature ovarian failure. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Long Name

Dachshund Homolog 2

Alternate Names

Dach2, dachshund homolog 2, dachshund homolog 2 (Drosophila), FLJ31391, MGC138545

Entrez Gene IDs

117154 (Human); 93837 (Mouse)

Gene Symbol

DACH2

UniProt

NP_444511.1 (Human)

Additional DACH2 Products

Product Documents for DACH2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for DACH2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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