DAK Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84142

Novus Biologicals, part of Bio-Techne
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NBP1-84142
NBP1-84142-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DAK Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SVPGSKPTFELSADEVELGLGIHGEAGVRRIKMATADEIVKLMLDHMTNTTNASHVPVQPGSSVVMMVNNLGGLSFLELGIIADATVRSLEGRGVKIARA

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (81%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DAK Antibody

Immunohistochemistry-Paraffin: DAK Antibody [NBP1-84142]

Immunohistochemistry-Paraffin: DAK Antibody [NBP1-84142]

Immunohistochemistry-Paraffin: DAK Antibody [NBP1-84142] - Staining in human duodenum and skeletal muscle tissues using anti-TKFC antibody. Corresponding TKFC RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: DAK Antibody [NBP1-84142]

Immunohistochemistry-Paraffin: DAK Antibody [NBP1-84142]

Immunohistochemistry-Paraffin: DAK Antibody [NBP1-84142] - Staining of human duodenum, kidney, liver and skeletal muscle using Anti-TKFC antibody NBP1-84142 (A) shows similar protein distribution across tissues to independent antibody NBP2-48894 (B).
Western Blot: DAK Antibody [NBP1-84142]

Western Blot: DAK Antibody [NBP1-84142]

Western Blot: DAK Antibody [NBP1-84142] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
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Applications for DAK Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:5000 - 1:10000

Immunohistochemistry-Paraffin

1:5000 - 1:10000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DAK

Alternate Names

ATP-dependent dihydroxyacetone kinase, bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing), DHA kinase, Dha kinase/FMN cyclase, dihydroxyacetone kinase 2 homolog (S. cerevisiae), dihydroxyacetone kinase 2 homolog (yeast), DKFZP586B1621, FAD-AMP lyase cyclic FMN forming, FAD-AMP lyase cyclizing, glycerone kinase, MGC5621, NET45

Gene Symbol

DAK

Additional DAK Products

Product Documents for DAK Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DAK Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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