DAP Kinase 1 Antibody (12B2-R)

Western Blot: DAP Kinase 1 Antibody (12B2-R) [NBP3-32256]

Western Blot: DAP Kinase 1 Antibody (12B2-R) [NBP3-32256] - Western blot analysis of DAP Kinase 1 on different lysates with Mouse anti-DAP Kinase 1 antibody (NBP3-32256) at 1/2,000 dilution.

Lane 1: A549 cell lysate
Lane 2: TF-1 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: Human brain tissue lysate

Lysates/proteins at 30 ug/Lane.

Predicted band size: 160 kDa
Observed band size: 160 kDa

Exposure time: 2 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32256) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: DAP Kinase 1 Antibody (12B2-R) [NBP3-32256]

Immunohistochemistry: DAP Kinase 1 Antibody (12B2-R) [NBP3-32256] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-DAP Kinase 1 antibody (NBP3-32256) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32256) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.