DAP Kinase 1 Antibody (2E7)
Novus Biologicals, part of Bio-Techne | Catalog # H00001612-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Proximity Ligation Assay, Sandwich ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 2E7
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
DAPK1 (NP_004929, 1211 a.a. ~ 1310 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LLLDSVCSTIENVMATTLPGLLTVKHYLSPQQLREHHEPVMIYQPRDFFRAQTLKETSLTNTMGGYKESFSSIMCFGCHDVYSQASLGMDIHASDLNLLT
Specificity
DAPK1 - death-associated protein kinase 1
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for DAP Kinase 1 Antibody (2E7)
Sandwich ELISA: DAP Kinase 1 Antibody (2E7) [H00001612-M01] - Detection limit for recombinant GST tagged DAPK1 is approximately 0.1ng/ml as a capture antibody.
Proximity Ligation Assay: DAP Kinase 1 Antibody (2E7) [H00001612-M01]
Proximity Ligation Assay: DAP Kinase 1 Antibody (2E7) [H00001612-M01] - Analysis of protein-protein interactions between MAPK3 and DAPK1. HeLa cells were stained with anti-MAPK3 rabbit purified polyclonal 1:1200 and anti-DAPK1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).Applications for DAP Kinase 1 Antibody (2E7)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Proximity Ligation Assay
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against recombinant protein on ELISA. GST alone used as a negative control.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: DAP Kinase 1
Long Name
Death-associated protein kinase 1
Alternate Names
DAPK, DAPK1
Entrez Gene IDs
1612 (Human)
Gene Symbol
DAPK1
OMIM
600831 (Human)
UniProt
Additional DAP Kinase 1 Products
Product Documents for DAP Kinase 1 Antibody (2E7)
Product Specific Notices for DAP Kinase 1 Antibody (2E7)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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