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DAP Kinase 1 Antibody (2E7)

Novus Biologicals, part of Bio-Techne | Catalog # H00001612-M01

Novus Biologicals, part of Bio-Techne
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H00001612-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Proximity Ligation Assay, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2E7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

DAPK1 (NP_004929, 1211 a.a. ~ 1310 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LLLDSVCSTIENVMATTLPGLLTVKHYLSPQQLREHHEPVMIYQPRDFFRAQTLKETSLTNTMGGYKESFSSIMCFGCHDVYSQASLGMDIHASDLNLLT

Specificity

DAPK1 - death-associated protein kinase 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for DAP Kinase 1 Antibody (2E7)

Sandwich ELISA: DAP Kinase 1 Antibody (2E7) [H00001612-M01] - Detection limit for recombinant GST tagged DAPK1 is approximately 0.1ng/ml as a capture antibody.
Proximity Ligation Assay: DAP Kinase 1 Antibody (2E7) [H00001612-M01]

Proximity Ligation Assay: DAP Kinase 1 Antibody (2E7) [H00001612-M01]

Proximity Ligation Assay: DAP Kinase 1 Antibody (2E7) [H00001612-M01] - Analysis of protein-protein interactions between MAPK3 and DAPK1. HeLa cells were stained with anti-MAPK3 rabbit purified polyclonal 1:1200 and anti-DAPK1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Applications for DAP Kinase 1 Antibody (2E7)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Proximity Ligation Assay

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against recombinant protein on ELISA. GST alone used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DAP Kinase 1

Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate.

Long Name

Death-associated protein kinase 1

Alternate Names

DAPK, DAPK1

Entrez Gene IDs

1612 (Human)

Gene Symbol

DAPK1

OMIM

600831 (Human)

Additional DAP Kinase 1 Products

Product Documents for DAP Kinase 1 Antibody (2E7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DAP Kinase 1 Antibody (2E7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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