DAP Kinase 2 Antibody (S09-8F2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-19618

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-19618-100ul
NBP3-19618-50ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S09-8F2

Concentration

0.3 mg/ml

View all DAPK2 Antibodies »

Product Summary for DAP Kinase 2 Antibody (S09-8F2)

Immunogen

A synthetic peptide of human DAP Kinase 2 (Uniprot # Q9UIK4)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DAP Kinase 2 Antibody (S09-8F2)

Western Blot: DAP Kinase 2 Antibody (S09-8F2) [NBP3-19618]

Western Blot: DAP Kinase 2 Antibody (S09-8F2) [NBP3-19618]

Western Blot: DAP Kinase 2 Antibody (S09-8F2) [NBP3-19618] - Western blot detection of DAP Kinase 2 in Rat spleen cell lysates using NBP3-19618 (1:1000 diluted). Predicted band size: 43kDa. Observed band size: 43kDa.

Applications for DAP Kinase 2 Antibody (S09-8F2)

Application
Recommended Usage

Western Blot

1/1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DAPK2

Protein kinase termed death-associated protein kinase 2 (DAPK2) dependant on calcium/calmodulin (Ca2+/CaM) contains an N-terminal protein kinase domain followed by a conserved CaM-binding domain with significant homologies to those of DAP kinase, a protein kinase involved in apoptosis. Overexpression of DAPK2 significantly induced the morphological changes characteristic of apoptosis. Results indicate that DAPK2 is an additional member of DAP kinase family involved in apoptotic signaling (1). The region of homology spans the catalytic domain and the CaM-regulatory region, whereas the remaining C-terminal part of the protein differs completely from DAP kinase and displays no homology to any known protein. The catalytic domain is also homologous to the recently identified ZIP kinase and to a lesser extent to the catalytic domains of DRAK1 and -2 (2). DAPK2 has a novel regulatory mechanism that controls its pro-apoptotic functions. It comprises a single autophosphorylation event mapped to Ser308 within the CaM regulatory domain. A negative charge at this site reduces both the binding to CaM and the formation of DRP-1 homodimers (3).

Long Name

Death-associated Protein Kinase 2

Alternate Names

DAP Kinase 2, DRP-1

Gene Symbol

DAPK2

Additional DAPK2 Products

Product Documents for DAP Kinase 2 Antibody (S09-8F2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DAP Kinase 2 Antibody (S09-8F2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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