DAP Kinase 2 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-02982

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all DAPK2 Antibodies »

Product Summary for DAP Kinase 2 Antibody - Azide and BSA Free

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DAP Kinase 2 (NP_055141.2). MFQASMRSPNMEPFKQQKVEDFYDIGEELGSGQFAIVKKCREKSTGLEYAAKFIKKRQSRASRRGVSREEIEREVSILRQVLHHNVITLHDVYENRTDVV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DAP Kinase 2 Antibody - Azide and BSA Free

Western Blot: DAP Kinase 2 AntibodyAzide and BSA Free [NBP3-02982]

Western Blot: DAP Kinase 2 AntibodyAzide and BSA Free [NBP3-02982]

Western Blot: DAP Kinase 2 Antibody [NBP3-02982] - Western blot analysis of extracts of Rat skeletal muscle, using DAP Kinase 2 antibody (NBP3-02982) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Immunocytochemistry/ Immunofluorescence: DAP Kinase 2 Antibody - Azide and BSA Free [NBP3-02982]

Immunocytochemistry/ Immunofluorescence: DAP Kinase 2 Antibody - Azide and BSA Free [NBP3-02982]

Immunocytochemistry/Immunofluorescence: DAP Kinase 2 Antibody [NBP3-02982] - Immunofluorescence analysis of U2OS cells using DAP Kinase 2 Rabbit pAb (NBP3-02982) at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: DAP Kinase 2 Antibody - Azide and BSA Free [NBP3-02982]

Immunocytochemistry/ Immunofluorescence: DAP Kinase 2 Antibody - Azide and BSA Free [NBP3-02982]

Immunocytochemistry/Immunofluorescence: DAP Kinase 2 Antibody [NBP3-02982] - Immunofluorescence analysis of NIH/3T3 cells using DAP Kinase 2 Rabbit pAb (NBP3-02982) at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining.
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Applications for DAP Kinase 2 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

Azide and BSA Free

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DAPK2

Protein kinase termed death-associated protein kinase 2 (DAPK2) dependant on calcium/calmodulin (Ca2+/CaM) contains an N-terminal protein kinase domain followed by a conserved CaM-binding domain with significant homologies to those of DAP kinase, a protein kinase involved in apoptosis. Overexpression of DAPK2 significantly induced the morphological changes characteristic of apoptosis. Results indicate that DAPK2 is an additional member of DAP kinase family involved in apoptotic signaling (1). The region of homology spans the catalytic domain and the CaM-regulatory region, whereas the remaining C-terminal part of the protein differs completely from DAP kinase and displays no homology to any known protein. The catalytic domain is also homologous to the recently identified ZIP kinase and to a lesser extent to the catalytic domains of DRAK1 and -2 (2). DAPK2 has a novel regulatory mechanism that controls its pro-apoptotic functions. It comprises a single autophosphorylation event mapped to Ser308 within the CaM regulatory domain. A negative charge at this site reduces both the binding to CaM and the formation of DRP-1 homodimers (3).

Long Name

Death-associated Protein Kinase 2

Alternate Names

DAP Kinase 2, DRP-1

Gene Symbol

DAPK2

Additional DAPK2 Products

Product Documents for DAP Kinase 2 Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for DAP Kinase 2 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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