DAP3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56325

Novus Biologicals, part of Bio-Techne
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NB100-56325
NB100-56325SS
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all DAP3 Antibodies »

Product Specifications

Immunogen

The antibody was raised against a synthetic peptide corresponding to amino acids 44-57 (TSDSDPAKHGEQHE) of mouse DAP-3 protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DAP3 Antibody

Western Blot: DAP3 Antibody [NB100-56325]

Western Blot: DAP3 Antibody [NB100-56325]

Western Blot: DAP3 Antibody [NB100-56325] - analysis of 10 ug of total cell lysate from Jurkat cells with this antibody.

Applications for DAP3 Antibody

Application
Recommended Usage

Western Blot

1-2 ug/ml
Application Notes
In Jurkat, a 45 kDa band is observed.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DAP3

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Recently, five novel apoptotic genes (named DAP genes for: Death Associated Proteins) have been identified that code for proteins, which display a diverse spectrum of biochemical activities. These include a novel type of calcium/calmodulin-regulated kinase which carries ankyrin repeats and a death domain (DAP-kinase), a nucleotide-binding protein (DAP-3), a small proline-rich cytoplasmic protein (DAP-1), and a novel homolog of the eIF4G translation initiation factor (DAP-5). DAP-3 has an ATP/GTP binding motive (P-loop) and has been isolated from human and mouse and named as hDAP-3 and mDAP-3 (Death Associated Protein-3), respectively. Sequence analysis revealed an 82% amino acid identity between human and mouse protein. In situ hybridization and northern blot analysis showed an abundant mRNA expression with a pronounced expression in highly proliferative epithelial compartments. Overexpression of DAP-3 leads to cytochrome c release and induction of cell death. DAP-3 mediated apoptosis was shown to depend on a functional P-loop. mDAP-3 is localized to the mitochondrial matrix and in contrast to cytochrome c, retained its mitochondrial localization during apoptosis induction.

Alternate Names

bMRP-10, DAP-3Death-associated protein 3, death associated protein 3, DKFZp686G12159,28S ribosomal protein S29, mitochondrial, FLJ12817, MGC126058, MGC126059, mitochondrial 28S ribosomal protein S29, MRP-S29Ionizing radiation resistance conferring protein, MRPS29S29mt

Entrez Gene IDs

7818 (Human); 65111 (Mouse); 295238 (Rat)

Gene Symbol

DAP3

UniProt

P51398 (Human)

Additional DAP3 Products

Product Documents for DAP3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DAP3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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