DARC Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-2421
![Immunohistochemistry-Paraffin: DARC Antibody [NB100-2421]](https://resources.bio-techne.com/images/products/DARC-Antibody-Immunohistochemistry-Paraffin-NB100-2421-img0008.jpg "Immunohistochemistry-Paraffin: DARC Antibody [NB100-2421]")
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Cited:
Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence HRAELSPSTENSSQLDFED-C corresponding to N-Terminus according to NP_001116423.1, NP_002027.2.
Specificity
This antibody is expected to recognize both reported isoforms (NP_001116423.1; NP_002027.2).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for DARC Antibody
![Western Blot: DARC Antibody [NB100-2421]](https://resources.bio-techne.com/images/products/DARC-Antibody-Western-Blot-NB100-2421-img0007.jpg "Western Blot: DARC Antibody [NB100-2421]")
Western Blot: DARC Antibody [NB100-2421]
Western Blot: DARC Antibody [NB100-2421] - DARC (0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.![Immunohistochemistry-Paraffin: DARC Antibody [NB100-2421]](https://resources.bio-techne.com/images/products/DARC-Antibody-Immunohistochemistry-Paraffin-NB100-2421-img0009.jpg "Immunohistochemistry-Paraffin: DARC Antibody [NB100-2421]")
Immunohistochemistry-Paraffin: DARC Antibody [NB100-2421]
Immunohistochemistry-Paraffin: DARC Antibody [NB100-2421] - Staining of paraffin embedded Human Lung. Microwaved antigen retrieval with citrate buffer Ph 6, AP-staining.![Flow Cytometry: DARC Antibody [NB100-2421]](https://resources.bio-techne.com/images/products/DARC-Antibody-Flow-Cytometry-NB100-2421-img0010.jpg "Flow Cytometry: DARC Antibody [NB100-2421]")
Flow Cytometry: DARC Antibody [NB100-2421]
Flow Cytometry: DARC Antibody [NB100-2421] - analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (0.4ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.Applications for DARC Antibody
Application
Recommended Usage
Flow Cytometry
1:1000
Immunohistochemistry
3 ug/ml
Immunohistochemistry-Paraffin
3 ug/ml
Peptide ELISA
detection limit 1:16000
Western Blot
0.3-1 ug/ml
Application Notes
WB: Approx 37kDa band observed in Human Kidney and Liver lysates (calculated MW of 35.6kDa according to NP_002027.2).
IHC-P: In paraffin embedded human kidney stains epithelial cells of renal tubules and endothelial cells of vessels. The granular staining is compatible with the endoplasmic reticulum association of the protein. This antibody has also been successfully used on Human Lung showing endothelial staining.
Flow cytometry Analysis of HEK293 cells. A customer has reported successful staining of HEK293S-TetR transfected with DARC receptor at a dilution of 1/1000.
IHC-P: In paraffin embedded human kidney stains epithelial cells of renal tubules and endothelial cells of vessels. The granular staining is compatible with the endoplasmic reticulum association of the protein. This antibody has also been successfully used on Human Lung showing endothelial staining.
Flow cytometry Analysis of HEK293 cells. A customer has reported successful staining of HEK293S-TetR transfected with DARC receptor at a dilution of 1/1000.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: DARC
Long Name
Duffy Antigen, Receptor For Chemokines
Alternate Names
ACKR1, CD234, Duffy Antigen, Fy Antigen, GPD, GpFy
Entrez Gene IDs
2532 (Human)
Gene Symbol
ACKR1
UniProt
Additional DARC Products
Product Documents for DARC Antibody
Product Specific Notices for DARC Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...