DARPP-32 [p Thr34] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-224

Novus Biologicals, part of Bio-Techne
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NB300-224
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%), Chicken (100%), Primate (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all DARPP-32 Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr34 conjugated to KLH. Accession # Q6J4I0

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24316474). Human reactivity reported in scientific literature (PMID: 19301121).

Modification

p Thr34

Specificity

Specific for endogenous levels of the ~32 kDa DARPP-32 protein phosphorylated at Thr34. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.

Marker

Neuronal Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DARPP-32 [p Thr34] Antibody

Western Blot: DARPP-32 [p Thr34] Antibody [NB300-224]

Western Blot: DARPP-32 [p Thr34] Antibody [NB300-224]

Western Blot: DARPP-32 [p Thr34] Antibody [NB300-224]

Applications for DARPP-32 [p Thr34] Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Western Blot

1:1000
Application Notes
This antibody is useful for Western blot, where a band is seen at approximately 32 kDa. Immunohistochemistry was reported in scientific literature.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DARPP-32

DARPP-32, a dopamine (DA) and cAMP-regulated ~32k phosphoprotein, is associated with dopaminoceptive neurons bearing D-1 receptors in the basal ganglia. The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA. Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine, and Prozac.

Long Name

Dopamine and cAMP-regulated Phosphoprotein

Alternate Names

DARPP32, PPP1R1B

Entrez Gene IDs

84152 (Human); 19049 (Mouse); 360616 (Rat)

Gene Symbol

PPP1R1B

UniProt

Q6J4I0 (Rat)

Additional DARPP-32 Products

Product Documents for DARPP-32 [p Thr34] Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DARPP-32 [p Thr34] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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