DARPP-32 [p Thr75] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB300-234
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Mouse, Rat
Cited:
Human, Mouse
Predicted:
Bovine (100%), Canine (100%), Chicken (100%), Human (100%), Primate (100%). Backed by our 100% Guarantee.
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr75 conjugated to KLH. Accession # Q6J4I0
Modification
p Thr75
Localization
Cytoplasmic
Specificity
Specific for endogenous levels of the ~32 kDa DARPP-32 protein phosphorylated at Thr75. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.
Marker
Neuronal Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for DARPP-32 [p Thr75] Antibody
![Western Blot: DARPP-32 [p Thr75] Antibody [NB300-234]](https://resources.bio-techne.com/images/products/DARPP32-[p-Thr75]-Antibody-Western-Blot-NB300-234-img0003.jpg "Western Blot: DARPP-32 [p Thr75] Antibody [NB300-234]")
Western Blot: DARPP-32 [p Thr75] Antibody [NB300-234]
Western Blot: DARPP32 [p Thr75] Antibody [NB300-234] - Analysis of rat caudate lysate showing specific immunolabeling of the ~32k DARPP-32 phosphorylated at Thr75 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated (1200 units for 30 min) before being exposed to the Anti-Thr75 DARPP-32. The immunolabeling is completely eliminated by treatment.![Western Blot: DARPP-32 [p Thr75] Antibody [NB300-234]](https://resources.bio-techne.com/images/products/DARPP32-[p-Thr75]-Antibody-Western-Blot-NB300-234-img0002.jpg "Western Blot: DARPP-32 [p Thr75] Antibody [NB300-234]")
Western Blot: DARPP-32 [p Thr75] Antibody [NB300-234]
Western Blot: DARPP32 [p Thr75] Antibody [NB300-234] - Analysis of Rat caudate lysates prepared from caudate slices that had been incubated in the absence and presence of okadaic acid. The labeling by the antibody to DARPP-32 Thr75 is markedly increased by the okadaic acid treatment.Applications for DARPP-32 [p Thr75] Antibody
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: DARPP-32
Long Name
Dopamine and cAMP-regulated Phosphoprotein
Alternate Names
DARPP32, PPP1R1B
Gene Symbol
PPP1R1B
UniProt
Additional DARPP-32 Products
Product Documents for DARPP-32 [p Thr75] Antibody
Product Specific Notices for DARPP-32 [p Thr75] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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