DARPP-32 [p Ser137] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29514

Novus Biologicals, part of Bio-Techne
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NBP2-29514
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Predicted:

Bovine (100%), Canine (100%), Chicken (100%), Human (100%), Primate (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all DARPP-32 Antibodies »

Product Summary for DARPP-32 [p Ser137] Antibody

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser137 conjugated to KLH. Accession # Q6J4I0

Modification

p Ser137

Specificity

Specific for endogenous levels of the ~32 kDa DARPP-32 protein phosphorylated at Ser137. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Marker

Neuronal Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DARPP-32 [p Ser137] Antibody

Western Blot: DARPP-32 [p Ser137] Antibody [NBP2-29514]

Western Blot: DARPP-32 [p Ser137] Antibody [NBP2-29514]

Western Blot: DARPP32 [p Ser137] Antibody [NBP2-29514] - Western blot of rat caudate lysate showing specific immunolabeling of the ~32k DARPP-32 phosphorylated at Ser137 (Control). Phosphospecificity is shown in the second lane where immunolabeling is blocked by preadsorption of the phospho-peptide used as antigen (peptide) but not by the corresponding dephosphopeptide (not shown).

Applications for DARPP-32 [p Ser137] Antibody

Application
Recommended Usage

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DARPP-32

DARPP-32 is a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac® (Maldve et al., 2002; Lindskog et al., 2002; Svenningsson et al., 2002). Serine 137 is phosphorylated by casein kinase 1 and phosphorylation of this site is increased following acute administration of Prozac® (Svenningsson et al., 2002).

Long Name

Dopamine and cAMP-regulated Phosphoprotein

Alternate Names

DARPP32, PPP1R1B

Entrez Gene IDs

360616 (Rat)

Gene Symbol

PPP1R1B

UniProt

Q6J4I0 (Rat)

Additional DARPP-32 Products

Product Documents for DARPP-32 [p Ser137] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for DARPP-32 [p Ser137] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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