Daxx Antibody (CL3580)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61622

Novus Biologicals, part of Bio-Techne
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NBP2-61622
NBP2-61622-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL3580

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Daxx Antibodies »

Product Summary for Daxx Antibody (CL3580)

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: ARGSSSSGGKKCYKLENEKLFEEFLELCKMQTADHPEVVPFLYNRQQRAHSLFLASAEFCNILSRVLSRARSRPAKLYVYINELCTVLKAHSAKKKLNLAPAATTSNEPSGNNPPTHLSLDPTNAENTASQSPRTR

Epitope

PEVVPFLYNR

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Daxx Antibody (CL3580)

Immunocytochemistry/ Immunofluorescence: Daxx Antibody (CL3580) [NBP2-61622]

Immunocytochemistry/ Immunofluorescence: Daxx Antibody (CL3580) [NBP2-61622]

Immunocytochemistry/Immunofluorescence: Daxx Antibody (CL3580) [NBP2-61622] - Staining of HeLa cells using the Anti-DAXX monoclonal antibody, showing specific staining in the nucleoplasm and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: Daxx Antibody (CL3580) [NBP2-61622]

Immunohistochemistry-Paraffin: Daxx Antibody (CL3580) [NBP2-61622]

Immunohistochemistry-Paraffin: Daxx Antibody (CL3580) [NBP2-61622] - Staining of human skeletal muscle shows absence of immunoreactivity (negative control).
Immunohistochemistry: Daxx Antibody (CL3580) [NBP2-61622]

Immunohistochemistry: Daxx Antibody (CL3580) [NBP2-61622]

Immunohistochemistry: Daxx Antibody (CL3580) [NBP2-61622] - Staining of human cervix shows moderate nuclear immunoreactivity in epithelial and underlying connective tissue cells.
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Applications for Daxx Antibody (CL3580)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Daxx

Daxx (death-domain-associated protein) was originally identified as a cytoplasmic protein that binds to the death domain of the transmembrane death receptor Fas. It is now that known that the a large porportion of Daxx molecules are nuclear and associate with the promyelocytic leukaemia nuclear body (PML-NB) and other subnuclear domains (reviewed in Salomoni and Khelifi). The promyelocytic leukemia protein Pml is an essential component of the PML-NB. Data suggests that certain stimuli including Fas stimulation, causes Daxx to be translocated from the nucleus to the cytoplasm. Daxx is ubiquitously expressed, and particularly high levels of Daxx have been reported in the thymus and testes. At the cellular level Daxx may be found in cytoplasm and within heterochromatic regions of the nucleus. Daxx has been reported to interact and co-localize with Pml in the nucleus of tumor cell lines and primary cells. Pml is necessary for Fas-induced cell death and Daxx pro-apoptotic functions. Daxx is thought to have both pro- and anti-apoptotic functions depending on the stimulus and the cell type. Daxx pro-apoptotic functions appear to occur in both the cytoplasm and nucleus. Although the mechanisms remain to be fully elucidated, research indicates that Daxx plays a role in the pathology of human diseases including cancer and neurodegerative disorders. Human Daxx is a 740 amino acid protein (GenBank no. gi/62898369).

Long Name

Fas Death Domain-associated Protein

Alternate Names

BING2, DAP6

Gene Symbol

DAXX

Additional Daxx Products

Product Documents for Daxx Antibody (CL3580)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Daxx Antibody (CL3580)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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