DAZ1 Antibody (3E3)

Novus Biologicals, part of Bio-Techne | Catalog # H00001617-M06

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3E3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DAZ1 Antibodies »

Product Specifications

Immunogen

DAZ1 (AAH18119, 21 a.a. ~ 120 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SSSAAASQGWVLPEGKIVPNTVFVGGIDARMDETEIGSCFGRYGSVKEVKIITNRTGVSKGYGFVSFVNDVDVQKIVGSQIHFHGKKLKLGPAIRKQKLC

Specificity

DAZ1 - deleted in azoospermia 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for DAZ1 Antibody (3E3)

Western Blot: DAZ1 Antibody (3E3) [H00001617-M06]

Western Blot: DAZ1 Antibody (3E3) [H00001617-M06]

Western Blot: DAZ1 Antibody (3E3) [H00001617-M06] - Analysis of DAZ1 expression in Daoy.
Immunohistochemistry-Paraffin: DAZ1 Antibody (3E3) [H00001617-M06]

Immunohistochemistry-Paraffin: DAZ1 Antibody (3E3) [H00001617-M06]

Immunohistochemistry-Paraffin: DAZ1 Antibody (3E3) [H00001617-M06] - Analysis of monoclonal antibody to DAZ1 on formalin-fixed paraffin-embedded human testis. Antibody concentration 1 ug/ml
ELISA: DAZ1 Antibody (3E3) [H00001617-M06]

ELISA: DAZ1 Antibody (3E3) [H00001617-M06]

ELISA: DAZ1 Antibody (3E3) [H00001617-M06] - Detection limit for recombinant GST tagged DAZ1 is approximately 0.1ng/ml as a capture antibody.

Applications for DAZ1 Antibody (3E3)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DAZ1

This gene is a member of the DAZ gene family and is a candidate for the human Y-chromosomal azoospermia factor (AZF). Its expression is restricted to premeiotic germ cells, particularly in spermatogonia. It encodes an RNA-binding protein that is important for spermatogenesis. Four copies of this gene are found on chromosome Y within palindromic duplications; one pair of genes is part of the P2 palindrome and the second pair is part of the P1 palindrome. Each gene contains a 2.4 kb repeat including a 72-bp exon, called the DAZ repeat; the number of DAZ repeats is variable and there are several variations in the sequence of the DAZ repeat. Each copy of the gene also contains a 10.8 kb region that may be amplified; this region includes five exons that encode an RNA recognition motif (RRM) domain. This gene contains three copies of the 10.8 kb repeat. However, no transcripts containing three copies of the RRM domain have been described; thus the RefSeq for this gene contains only two RRM domains.

Alternate Names

deleted in azoospermia, deleted in azoospermia 1, FLJ17175, SPGYDAZdeleted in azoospermia protein 1

Entrez Gene IDs

1617 (Human)

Gene Symbol

DAZ1

OMIM

400003 (Human)

UniProt

NP_001006118.2 (Human)

Additional DAZ1 Products

Product Documents for DAZ1 Antibody (3E3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DAZ1 Antibody (3E3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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