DAZ1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98991

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all DAZ1 Antibodies »

Product Summary for DAZ1 Antibody

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human DAZ1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for DAZ1 Antibody

Immunocytochemistry/ Immunofluorescence: DAZ1 Antibody [NBP2-98991]

Immunocytochemistry/ Immunofluorescence: DAZ1 Antibody [NBP2-98991]

Immunocytochemistry/Immunofluorescence: DAZ1 Antibody [NBP2-98991] - Immunofluorescence staining of DAZ1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human DAZ1 polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.
Immunohistochemistry-Paraffin: DAZ1 Antibody [NBP2-98991]

Immunohistochemistry-Paraffin: DAZ1 Antibody [NBP2-98991]

Immunohistochemistry-Paraffin: DAZ1 Antibody [NBP2-98991] - Immunochemical staining of human DAZ1 in human testis with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Immunohistochemistry-Paraffin: DAZ1 Antibody [NBP2-98991]

Immunohistochemistry-Paraffin: DAZ1 Antibody [NBP2-98991]

Immunohistochemistry-Paraffin: DAZ1 Antibody [NBP2-98991] - Immunochemical staining of human DAZ1 in human liver with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).

Applications for DAZ1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Immunohistochemistry-Paraffin

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DAZ1

DAZ1 is a member of the DAZ gene family and is a candidate for the human Y-chromosomal azoospermia factor (AZF). Its expression is restricted to premeiotic germ cells, particularly in spermatogonia. It encodes an RNA-binding protein that is important for spermatogenesis. Four copies of this gene are found on chromosome Y within palindromic duplications; one pair of genes is part of the P2 palindrome and the second pair is part of the P1 palindrome. Each gene contains a 2.4 kb repeat including a 72-bp exon, called the DAZ repeat; the number of DAZ repeats is variable and there are several variations in the sequence of the DAZ repeat. Each copy of the gene also contains a 10.8 kb region that may be amplified; this region includes five exons that encode an RNA recognition motif (RRM) domain. This gene contains three copies of the 10.8 kb repeat. However, no transcripts containing three copies of the RRM domain have been described; thus the RefSeq for this gene contains only two RRM domains.

Alternate Names

deleted in azoospermia, deleted in azoospermia 1, FLJ17175, SPGYDAZdeleted in azoospermia protein 1

Gene Symbol

DAZ1

Additional DAZ1 Products

Product Documents for DAZ1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for DAZ1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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