Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Orangutan (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

1.0 mg/ml

View all DBC1/p30 Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 873 to 923 of human p30 DBC Protein using the numbering given in entry NP_066997.3 (GeneID 57805).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for DBC1/p30 Antibody

Western Blot: DBC1/p30 Antibody [NBP2-22294]

Western Blot: DBC1/p30 Antibody [NBP2-22294]

Western Blot: DBC1/p30 Antibody [NBP2-22294] - Whole cell lysate (50 ug) from NIH3T3 , Renca, and TCMK-1 cells. NBP2-22294 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry-Paraffin: DBC1/p30 Antibody [NBP2-22294]

Immunohistochemistry-Paraffin: DBC1/p30 Antibody [NBP2-22294]

Immunohistochemistry-Paraffin: DBC1/p30 Antibody [NBP2-22294] - Section of mouse teratoma. Antibody: Affinity purified rabbit anti- BC1/p30 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).
Western Blot: DBC1/p30 Antibody [NBP2-22294]

Western Blot: DBC1/p30 Antibody [NBP2-22294]

Western Blot: DBC1/p30 Antibody [NBP2-22294] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), and Jurkat (50 ug) cells. NBP2-22294 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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Applications for DBC1/p30 Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:5000

Immunohistochemistry-Paraffin

1:1000 - 1:5000

Immunoprecipitation

2 - 10 ug/mg

Western Blot

1:2000 - 1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DBC1/p30

DBC1 inhibits SIRT1 deacetylase activity leading to increasing levels of p53/TP53 acetylation and p53-mediatedapoptosis. Inhibits SUV39H1 methyltransferase activity

Alternate Names

DBC.1, DBC1deleted in breast cancer 1, DBC-1p30 DBC protein, Deleted in breast cancer gene 1 protein, KIAA1967, NET35, p30 DBC, p30DBC

Gene Symbol

CCAR2

UniProt

NP_066997.3 (Human)

Additional DBC1/p30 Products

Product Documents for DBC1/p30 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for DBC1/p30 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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