DBF4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78282

Novus Biologicals, part of Bio-Techne
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NBP1-78282
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Key Product Details

Species Reactivity

Mouse, Rat

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all DBF4 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DKKRTEYLPAHEDRT corresponding to internal region according to NP_001177646.1.

Specificity

This antibody is expected to recognize both reported isoforms (NP_038754.1; NP_001177646.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for DBF4 Antibody

Western Blot: DBF4 Antibody [NBP1-78282]

Western Blot: DBF4 Antibody [NBP1-78282]

Western Blot: DBF4 Antibody [NBP1-78282] - (1ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for DBF4 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 70 kDa band observed in mouse and rat testis and thymus lysates (calculated MW of 74.2 kDa band according to NP_038754.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DBF4

Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle

Alternate Names

ASKchif, DBF4 homolog (S. cerevisiae), DBF4ACHIF, DBF4-type zinc finger-containing protein 1, ZDBF1DBF-type containing 1

Entrez Gene IDs

27214 (Mouse); 312046 (Rat)

Gene Symbol

DBF4

Additional DBF4 Products

Product Documents for DBF4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DBF4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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