DBPA Antibody (PCRP-YBX3-2D12) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24275
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # PCRP-YBX3-2D12
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for DBPA Antibody (PCRP-YBX3-2D12) - Azide and BSA Free
Immunogen
Recombinant full-length human DBPA protein
Localization
Nucleus. Cytoplasm.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Description
Antibody without azide - store at -20 to -80C.
Scientific Data Images for DBPA Antibody (PCRP-YBX3-2D12) - Azide and BSA Free
Protein Array: DBPA Antibody (PCRP-YBX3-2D12) - Azide and BSA Free [NBP3-24275] -
Analysis of Protein Array containing more than 19,000 full-length human proteins using DBPA antibody (PCRP-YBX3-2D12) - Azide and BSA Free
Immunohistochemistry-Paraffin: DBPA Antibody (PCRP-YBX3-2D12) - Azide and BSA Free [NBP3-24275] -
Formalin-fixed, paraffin-embedded human bladder carcinoma stained with DBPA antibody (PCRP-YBX3-2D12) - Azide and BSA Free. HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
Flow Cytometry: DBPA Antibody (PCRP-YBX3-2D12) - Azide and BSA Free [NBP3-24275] -
Flow cytometric analysis of PFA-fixed HeLa cells. DBPA antibody (PCRP-YBX3-2D12) - Azide and BSA Free followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).Applications for DBPA Antibody (PCRP-YBX3-2D12) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1-2ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2ug/ml
Immunohistochemistry-Paraffin
1-2ug/ml
Immunoprecipitation
1-2ug/100-500ug protein
Western Blot
1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: DBPA
Alternate Names
cold shock domain protein A, Cold shock domain-containing protein A, cold-shock domain protein A, CSDA1, dbpA, DBPAcold-shock domain containing A1, DNA-binding protein A, Single-strand DNA-binding protein NF-GMB, ZO-1-associated nucleic acid-binding protein, ZONAB
Gene Symbol
YBX3
Additional DBPA Products
Product Documents for DBPA Antibody (PCRP-YBX3-2D12) - Azide and BSA Free
Product Specific Notices for DBPA Antibody (PCRP-YBX3-2D12) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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