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DBR1 Antibody (3A7)

Novus Biologicals, part of Bio-Techne | Catalog # H00051163-M01

Novus Biologicals, part of Bio-Techne
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H00051163-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3A7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

DBR1 (NP_057300.2, 445 a.a. ~ 538 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SGMNTPSVEPSDQASEFSASFSDVRILPGSMIVSSDDTVDSTIDREGKPGGTVESGNGEDLTKVPLKRLSDEHEPEQRKKIKRRNQAIYAAVDD

Specificity

DBR1 - debranching enzyme homolog 1 (S. cerevisiae) (3A7)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for DBR1 Antibody (3A7)

Western Blot: DBR1 Antibody (3A7) [H00051163-M01]

Western Blot: DBR1 Antibody (3A7) [H00051163-M01]

Western Blot: DBR1 Antibody (3A7) [H00051163-M01] - Analysis of DBR1 expression in transfected 293T cell line by DBR1 monoclonal antibody (M01), clone 3A7.Lane 1: DBR1 transfected lysate(61.6 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: DBR1 Antibody (3A7) [H00051163-M01]

Immunocytochemistry/ Immunofluorescence: DBR1 Antibody (3A7) [H00051163-M01]

Immunocytochemistry/Immunofluorescence: DBR1 Antibody (3A7) [H00051163-M01] - Analysis of monoclonal antibody to DBR1 on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: DBR1 Antibody (3A7) [H00051163-M01] - Detection limit for recombinant GST tagged DBR1 is 0.03 ng/ml as a capture antibody.

Applications for DBR1 Antibody (3A7)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DBR1

The RNA lariat debranching enzyme, or DBR1, specifically hydrolyzes 2-prime-to-5-prime branched phosphodiester bonds at the branch point of excised lariat intron RNA and converts them into linear molecules.[supplied by OMIM]

Alternate Names

debranching enzyme (S. Cerevisiae) homolog 1, debranching enzyme homolog 1 (S. cerevisiae), EC 3.1, lariat debranching enzyme, RNA lariat debranching enzyme

Entrez Gene IDs

51163 (Human)

Gene Symbol

DBR1

Additional DBR1 Products

Product Documents for DBR1 Antibody (3A7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DBR1 Antibody (3A7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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