DBR1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85907

Novus Biologicals, part of Bio-Techne
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NBP1-85907
NBP1-85907-25ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DBR1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SALSSINPDEIMLDEEEDEDSIVSAHSGMNTPSVEPSDQASEFSASFSDVRILPGSMIVSSDDTVDSTIDREGKPGGTV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DBR1 Antibody

Western Blot: DBR1 Antibody [NBP1-85907]

Western Blot: DBR1 Antibody [NBP1-85907]

Western Blot: DBR1 Antibody [NBP1-85907] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: DBR1 Antibody [NBP1-85907]

Immunocytochemistry/ Immunofluorescence: DBR1 Antibody [NBP1-85907]

Immunocytochemistry/Immunofluorescence: DBR1 Antibody [NBP1-85907] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: DBR1 Antibody [NBP1-85907]

Immunohistochemistry-Paraffin: DBR1 Antibody [NBP1-85907]

Immunohistochemistry-Paraffin: DBR1 Antibody [NBP1-85907] - Staining of human stomach shows moderate to strong nuclear positivity in glandular cells.
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Applications for DBR1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DBR1

The RNA lariat debranching enzyme, or DBR1, specifically hydrolyzes 2-prime-to-5-prime branched phosphodiester bonds at the branch point of excised lariat intron RNA and converts them into linear molecules.[supplied by OMIM]

Alternate Names

debranching enzyme (S. Cerevisiae) homolog 1, debranching enzyme homolog 1 (S. cerevisiae), EC 3.1, lariat debranching enzyme, RNA lariat debranching enzyme

Gene Symbol

DBR1

Additional DBR1 Products

Product Documents for DBR1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DBR1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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