Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all DC8 Antibodies »

Product Summary for DC8 Antibody

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human DC8.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for DC8 Antibody

Western Blot: DC8 Antibody [NBP2-99483]

Western Blot: DC8 Antibody [NBP2-99483]

Western Blot: DC8 Antibody [NBP2-99483] - Anti-DC8 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 32 kDa. Observed band size: 34 kDa
Immunocytochemistry/ Immunofluorescence: DC8 Antibody [NBP2-99483]

Immunocytochemistry/ Immunofluorescence: DC8 Antibody [NBP2-99483]

Immunocytochemistry/Immunofluorescence: DC8 Antibody [NBP2-99483] - Immunofluorescence staining of DC8 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human DC8 polyclonal antibody (dilution ratio 1:5000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus .

Applications for DC8 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1500-1:50000

Western Blot

1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DC8

DC8 encodes a protein with two coiled-coil domains that localizes to kinetochores, which are chromosome-associated structures that attach to microtubules and mediate chromosome movements during cell division. It is part of a conserved protein complex that includes two chromodomain-containing proteins and a component of the outer plate of the kinetochore. This protein complex is proposed to bridge centromeric heterochromatin with the outer kinetochore structure. Multiple transcript variants encoding different isoforms have been found for this gene.

Alternate Names

C1orf48, chromosome 1 open reading frame 48, DC8, DKFZP566O1646, kinetochore-associated protein NSL1 homolog, MIS14, NSL1, MIND kinetochore complex component, homolog (S. cerevisiae)

Gene Symbol

NSL1

Additional DC8 Products

Product Documents for DC8 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for DC8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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