DCAF4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-05972

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all DCAF4 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human WD repeat-containing protein 21A using the numbering given in entry NP_056419.2 (GeneID 26094).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DCAF4 Antibody

Western Blot: DCAF4 Antibody [NBP1-05972]

Western Blot: DCAF4 Antibody [NBP1-05972]

Western Blot: DCAF4 Antibody [NBP1-05972] - Detection of Human and Mouse WDR21A by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-WDR21A antibody NBP1-05972 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunoprecipitation: DCAF4 Antibody [NBP1-05972]

Immunoprecipitation: DCAF4 Antibody [NBP1-05972]

Immunoprecipitation: DCAF4 Antibody [NBP1-05972] - Detection of human WDR21A by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-WDR21A antibody NBP1-05972 (lot NBP1-05972-2) used for IP at 6 ug per reaction. WDR21A was also immunoprecipitated by a previous lot of this antibody (lot NBP1-05972-1) and another rabbit anti-WDR21A antibody. For blotting immunoprecipitated WDR21A, NBP1-05972 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for DCAF4 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 5 µg/mg lysate

Western Blot

1:2000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DCAF4

This gene encodes a WD repeat-containing protein. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined. [provided by RefSeq]

Alternate Names

DDB1 and CUL4 associated factor 4, DDB1- and CUL4-associated factor 4, DKFZp434K114, MGC20547, MGC46524, WD repeat domain 21, WD repeat domain 21A, WD repeat-containing protein 21A, WDR21, WDR21A

Entrez Gene IDs

26094 (Human)

Gene Symbol

DCAF4

UniProt

NP_056419.2 (Human)

Additional DCAF4 Products

Product Documents for DCAF4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DCAF4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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