DCAF4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91820

Novus Biologicals, part of Bio-Techne
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NBP1-91820
NBP1-91820-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DCAF4 Antibodies »

Product Summary for DCAF4 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TSSGTAGTSSVPELPGFYFDPEKKRYFRLLPGHNNCNPLTKESIRQKEMESKRLRLLQEEDRRK

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%), Rat (80%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DCAF4 Antibody

Western Blot: DCAF4 Antibody [NBP1-91820]

Western Blot: DCAF4 Antibody [NBP1-91820]

Western Blot: DCAF4 Antibody [NBP1-91820] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: DCAF4 Antibody [NBP1-91820]

Immunocytochemistry/ Immunofluorescence: DCAF4 Antibody [NBP1-91820]

Immunocytochemistry/Immunofluorescence: DCAF4 Antibody [NBP1-91820] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: DCAF4 Antibody [NBP1-91820]

Immunohistochemistry-Paraffin: DCAF4 Antibody [NBP1-91820]

Immunohistochemistry-Paraffin: DCAF4 Antibody [NBP1-91820] - Staining of human uterus, pre-menopause shows strong cytoplasmic positivity in glandular cells.

Applications for DCAF4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DCAF4

DCAF4 encodes a WD repeat-containing protein. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined. [provided by RefSeq]

Alternate Names

DDB1 and CUL4 associated factor 4, DDB1- and CUL4-associated factor 4, DKFZp434K114, MGC20547, MGC46524, WD repeat domain 21, WD repeat domain 21A, WD repeat-containing protein 21A, WDR21, WDR21A

Gene Symbol

DCAF4

Additional DCAF4 Products

Product Documents for DCAF4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DCAF4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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