Dcp1a Antibody (3G4)

Immunocytochemistry/ Immunofluorescence: Dcp1a Antibody (3G4) [H00055802-M06]

Immunocytochemistry/Immunofluorescence: Dcp1a Antibody (3G4) [H00055802-M06] - The MIWI2/MAEL granule is a modified P-body. Localization of P-bodies in gonocytes. Cross section seminiferous tubule from wild-type testis (E18.5) were probed with antibodies against DCP1a. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pgen.1000764), licensed under a CC-BY license.

Immunocytochemistry/ Immunofluorescence: Dcp1a Antibody (3G4) [H00055802-M06]

Immunocytochemistry/Immunofluorescence: Dcp1a Antibody (3G4) [H00055802-M06] - Localization of P-bodies in gonocytes. Co-localization of MAEL and P-body component DCP1a in piP-bodies. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pgen.1000764), licensed under a CC-BY license.

Immunocytochemistry/ Immunofluorescence: Dcp1a Antibody (3G4) [H00055802-M06]

Immunocytochemistry/Immunofluorescence: Dcp1a Antibody (3G4) [H00055802-M06] - Anti-Dcp1alpha antibody recognizes its antigen. SCG neuron shown overexpressing GFP-Dcp1alpha. Note that all GFP-Dcp1alpha granules are recognized by anti-Dcp1alpha antibody. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/cddis2013297), licensed under a CC-BY license.

Immunocytochemistry/ Immunofluorescence: Dcp1a Antibody (3G4) [H00055802-M06]

Immunocytochemistry/Immunofluorescence: Dcp1a Antibody (3G4) [H00055802-M06] - Deconvoluted merged stacks of confocal microscopic images of typical neurons colabeled with anti-Dcp1alpha antibodies, the marker for the P-bodies. Note that the granules do not colocalize. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/cddis2013297), licensed under a CC-BY license.

ELISA: Dcp1a Antibody (3G4) [H00055802-M06]

ELISA: Dcp1a Antibody (3G4) [H00055802-M06] - Detection limit for recombinant GST tagged DCP1A is approximately 0.1ng/ml as a capture antibody.

Immunocytochemistry/ Immunofluorescence: Dcp1a Antibody (3G4) [H00055802-M06] -

Immunocytochemistry/ Immunofluorescence: Dcp1a Antibody (3G4) [H00055802-M06] - OTUD4 is recruited to stress granules. (A) Immunofluorescence of OTUD4 (shown in red) in SH-SY5Y cells that were either untreated, arsenite-treated (30 min, 0.5 mM) or heat-shocked (42°C, 1 h). Cells were co-stained for the stress granule marker protein TIA1 (green) & DAPI as a nuclear marker (blue). OTUD4 is redistributed to granular structures upon arsenite & heat-shock treatment. Granules also contain TIA1 & are considered as stress granules. Scale bar: 20 µm. The experiment was repeated two times. (B) Exogenously expressed FLAG–OTUD4 is recruited to stress granules in HeLa cells. Transfected cells were arsenite-treated (0.5 mM) for 40 min or left untreated & co-stained with anti-FLAG (red) & anti-G3BP1 antibodies (green). Nuclei are shown in blue (DAPI). Scale bar: 20 µm. The experiment was done at least three times. (C) OTUD4 granules do not colocalize with P-bodies. Shown is immunofluorescence of HeLa cells (untreated or 0.5 mM arsenite for 30 min) stained with anti-OTUD4 (red) & anti-DCP1a (green) antibodies. Scale bar: 20 µm. The experiment was performed two times. (D) OTUD4 granules contain mRNA. HeLa cells were treated with arsenite (0.5 mM) for 1 h. FISH was carried out with Cy3-labeled oligo(dT) (red), & cells were co-stained with anti-OTUD4 (shown in green). Scale bar: 20 µm. A representative image from four independent experiments is shown. (E) Scheme illustrating OTUD4 fragments used in F, numbers indicate amino acid borders of expression constructs. IDR, intrinsically disordered region. (F) HeLa cells were transfected with EGFP-tagged OTUD4 expression constructs as shown in E) & treated with arsenite (0.5 mM for 30 min) or left untreated to monitor intrinsic ability to form granules. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31138677), licensed under a CC-BY license. Not internally tested by Novus Biologicals.